Cargando…

A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library

BACKGROUND: Massive, parallel sequencing is a potent tool for dissecting the regulation of biological processes by revealing the dynamics of the cellular RNA profile under different conditions. Similarly, massive, parallel sequencing can be used to reveal the complexity of viral quasispecies that ar...

Descripción completa

Detalles Bibliográficos
Autores principales:	Waugh, Caryll, Cromer, Deborah, Grimm, Andrew, Chopra, Abha, Mallal, Simon, Davenport, Miles, Mak, Johnson
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403950/ https://www.ncbi.nlm.nih.gov/pubmed/25879746 http://dx.doi.org/10.1186/s12985-015-0280-x

Ejemplares similares

HIV-1 Mutation and Recombination Rates Are Different in Macrophages and T-cells
por: Cromer, Deborah, et al.
Publicado: (2016)

Display of a Maize cDNA library on baculovirus infected insect cells
por: Meller Harel, Helene Y, et al.
Publicado: (2008)

Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes
por: Nour-Eldin, Hussam H, et al.
Publicado: (2006)

Penalized likelihood for sparse contingency tables with an application to full-length cDNA libraries
por: Dahinden, Corinne, et al.
Publicado: (2007)

A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA
por: Davis, Claytus, et al.
Publicado: (2007)

Selective and flexible depletion of problematic sequences from RNA-seq libraries at the cDNA stage
por: Archer, Stuart K, et al.
Publicado: (2014)

Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNA
por: Yadav, Rajiv Kumar, et al.
Publicado: (2014)

Statistical method to compare massive parallel sequencing pipelines
por: Elsensohn, MH., et al.
Publicado: (2017)

COSMOS: Python library for massively parallel workflows
por: Gafni, Erik, et al.
Publicado: (2014)

Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA
por: Weiberg, Arne, et al.
Publicado: (2008)

Increased Throughput by Parallelization of Library Preparation for Massive Sequencing
por: Lundin, Sverker, et al.
Publicado: (2010)

Massively parallel nanowell-based single-cell gene expression profiling
por: Goldstein, Leonard D., et al.
Publicado: (2017)

The effect of column purification on cDNA indirect labelling for microarrays
por: Molas, M Lia, et al.
Publicado: (2007)

Application of massive parallel sequencing to whole genome SNP discovery in the porcine genome
por: Amaral, Andreia J, et al.
Publicado: (2009)

A new massively parallel nanoball sequencing platform for whole exome research
por: Xu, Yu, et al.
Publicado: (2019)

Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data
por: Zhou, Sun, et al.
Publicado: (2012)

The preparation of an infectious full-length cDNA clone of Saffold virus
por: Himeda, Toshiki, et al.
Publicado: (2011)

High throughput whole rumen metagenome profiling using untargeted massively parallel sequencing
por: Ross, Elizabeth M, et al.
Publicado: (2012)

Linear models enable powerful differential activity analysis in massively parallel reporter assays
por: Myint, Leslie, et al.
Publicado: (2019)

Efficient de novo assembly of large and complex genomes by massively parallel sequencing of Fosmid pools
por: Alexeyenko, Andrey, et al.
Publicado: (2014)

Automation of cDNA Synthesis and Labelling Improves Reproducibility
por: Klevebring, Daniel, et al.
Publicado: (2009)

cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries
por: Qi, Meifang, et al.
Publicado: (2021)

cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit
por: Chi, Jianxiang, et al.
Publicado: (2015)

Pseudo-Sanger sequencing: massively parallel production of long and near error-free reads using NGS technology
por: Ruan, Jue, et al.
Publicado: (2013)

Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting
por: Teer, Jamie K, et al.
Publicado: (2013)

Characterization of 954 bovine full-CDS cDNA sequences
por: Harhay, Gregory P, et al.
Publicado: (2005)

The full-ORF clone resource of the German cDNA Consortium
por: Bechtel, Stephanie, et al.
Publicado: (2007)

Quantification of massively parallel sequencing libraries – a comparative study of eight methods
por: Hussing, Christian, et al.
Publicado: (2018)

Massively parallel gene expression variation measurement of a synonymous codon library
por: Schmitz, Alexander, et al.
Publicado: (2021)

Accurately Measuring Recombination between Closely Related HIV-1 Genomes
por: Schlub, Timothy E., et al.
Publicado: (2010)

Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements
por: Branham, William S, et al.
Publicado: (2007)

Gibson assembly: an easy way to clone potyviral full-length infectious cDNA clones expressing an ectopic VPg
por: Bordat, Amandine, et al.
Publicado: (2015)

Screening of a Brassica napus bacterial artificial chromosome library using highly parallel single nucleotide polymorphism assays
por: Cao, Hieu Xuan, et al.
Publicado: (2013)

MASQOT: a method for cDNA microarray spot quality control
por: Bylesjö, Max, et al.
Publicado: (2005)

Operon information improves gene expression estimation for cDNA microarrays
por: Xiao, Guanghua, et al.
Publicado: (2006)

Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in Xenopus
por: Falk, Julien, et al.
Publicado: (2007)

Development of a cDNA array for chicken gene expression analysis
por: Burnside, Joan, et al.
Publicado: (2005)

Modelling malaria elimination on the internet
por: Maude, Richard J, et al.
Publicado: (2011)

Cloning and sequencing of murine T3 gamma cDNA from a subtractive cDNA library
Publicado: (1987)

Library Preparation and Multiplex Capture for Massive Parallel Sequencing Applications Made Efficient and Easy
por: Neiman, Mårten, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_sbab93frv60c5subuu3slelnu6