One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)

BACKGROUND: The pronuclear injection (PI) is the simplest and widely used method to generate transgenic (Tg) mice. Unfortunately, PI-based Tg mice show uncertain transgene expression due to random transgene insertion in the genome, usually with multiple copies. Thus, typically at least three or more...

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Detalles Bibliográficos
Autores principales: Ohtsuka, Masato, Miura, Hiromi, Mochida, Keiji, Hirose, Michiko, Hasegawa, Ayumi, Ogura, Atsuo, Mizutani, Ryuta, Kimura, Minoru, Isotani, Ayako, Ikawa, Masahito, Sato, Masahiro, Gurumurthy, Channabasavaiah B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404087/
https://www.ncbi.nlm.nih.gov/pubmed/25887549
http://dx.doi.org/10.1186/s12864-015-1432-5

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