Cargando…

Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi

Efficient acquisition and transmission of Borrelia burgdorferi by the tick vector, and the ability to persistently infect both vector and host, are important elements for the life cycle of the Lyme disease pathogen. Previous work has provided strong evidence implicating the significance of the vls l...

Descripción completa

Detalles Bibliográficos
Autores principales: Rogovskyy, Artem S., Casselli, Timothy, Tourand, Yvonne, Jones, Cami R., Owen, Jeb P., Mason, Kathleen L., Scoles, Glen A., Bankhead, Troy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404307/
https://www.ncbi.nlm.nih.gov/pubmed/25893989
http://dx.doi.org/10.1371/journal.pone.0124268
_version_ 1782367473884987392
author Rogovskyy, Artem S.
Casselli, Timothy
Tourand, Yvonne
Jones, Cami R.
Owen, Jeb P.
Mason, Kathleen L.
Scoles, Glen A.
Bankhead, Troy
author_facet Rogovskyy, Artem S.
Casselli, Timothy
Tourand, Yvonne
Jones, Cami R.
Owen, Jeb P.
Mason, Kathleen L.
Scoles, Glen A.
Bankhead, Troy
author_sort Rogovskyy, Artem S.
collection PubMed
description Efficient acquisition and transmission of Borrelia burgdorferi by the tick vector, and the ability to persistently infect both vector and host, are important elements for the life cycle of the Lyme disease pathogen. Previous work has provided strong evidence implicating the significance of the vls locus for B. burgdorferi persistence. However, studies involving vls mutant clones have thus far only utilized in vitro-grown or host-adapted spirochetes and laboratory strains of mice. Additionally, the effects of vls mutation on tick acquisition and transmission has not yet been tested. Thus, the importance of VlsE antigenic variation for persistent infection of the natural reservoir host, and for the B. burgdorferi enzootic life cycle in general, has not been examined to date. In the current work, Ixodes scapularis and Peromyscus maniculatus were infected with different vls mutant clones to study the importance of the vls locus for the enzootic cycle of the Lyme disease pathogen. The findings highlight the significance of the vls system for long-term infection of the natural reservoir host, and show that VlsE antigenic variability is advantageous for efficient tick acquisition of B. burgdorferi from the mammalian reservoir. The data also indicate that the adaptation state of infecting spirochetes influences B. burgdorferi avoidance from host antibodies, which may be in part due to its respective VlsE expression levels. Overall, the current findings provide the most direct evidence on the importance of VlsE for the enzootic cycle of Lyme disease spirochetes, and underscore the significance of VlsE antigenic variation for maintaining B. burgdorferi in nature.
format Online
Article
Text
id pubmed-4404307
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44043072015-05-02 Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi Rogovskyy, Artem S. Casselli, Timothy Tourand, Yvonne Jones, Cami R. Owen, Jeb P. Mason, Kathleen L. Scoles, Glen A. Bankhead, Troy PLoS One Research Article Efficient acquisition and transmission of Borrelia burgdorferi by the tick vector, and the ability to persistently infect both vector and host, are important elements for the life cycle of the Lyme disease pathogen. Previous work has provided strong evidence implicating the significance of the vls locus for B. burgdorferi persistence. However, studies involving vls mutant clones have thus far only utilized in vitro-grown or host-adapted spirochetes and laboratory strains of mice. Additionally, the effects of vls mutation on tick acquisition and transmission has not yet been tested. Thus, the importance of VlsE antigenic variation for persistent infection of the natural reservoir host, and for the B. burgdorferi enzootic life cycle in general, has not been examined to date. In the current work, Ixodes scapularis and Peromyscus maniculatus were infected with different vls mutant clones to study the importance of the vls locus for the enzootic cycle of the Lyme disease pathogen. The findings highlight the significance of the vls system for long-term infection of the natural reservoir host, and show that VlsE antigenic variability is advantageous for efficient tick acquisition of B. burgdorferi from the mammalian reservoir. The data also indicate that the adaptation state of infecting spirochetes influences B. burgdorferi avoidance from host antibodies, which may be in part due to its respective VlsE expression levels. Overall, the current findings provide the most direct evidence on the importance of VlsE for the enzootic cycle of Lyme disease spirochetes, and underscore the significance of VlsE antigenic variation for maintaining B. burgdorferi in nature. Public Library of Science 2015-04-20 /pmc/articles/PMC4404307/ /pubmed/25893989 http://dx.doi.org/10.1371/journal.pone.0124268 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Rogovskyy, Artem S.
Casselli, Timothy
Tourand, Yvonne
Jones, Cami R.
Owen, Jeb P.
Mason, Kathleen L.
Scoles, Glen A.
Bankhead, Troy
Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title_full Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title_fullStr Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title_full_unstemmed Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title_short Evaluation of the Importance of VlsE Antigenic Variation for the Enzootic Cycle of Borrelia burgdorferi
title_sort evaluation of the importance of vlse antigenic variation for the enzootic cycle of borrelia burgdorferi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404307/
https://www.ncbi.nlm.nih.gov/pubmed/25893989
http://dx.doi.org/10.1371/journal.pone.0124268
work_keys_str_mv AT rogovskyyartems evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT cassellitimothy evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT tourandyvonne evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT jonescamir evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT owenjebp evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT masonkathleenl evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT scolesglena evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi
AT bankheadtroy evaluationoftheimportanceofvlseantigenicvariationfortheenzooticcycleofborreliaburgdorferi