Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing

BACKGROUND: HLA genotyping by next generation sequencing (NGS) requires three basic steps, PCR, NGS, and allele assignment. Compared to the conventional methods, such as PCR-sequence specific oligonucleotide primers (SSOP) and -sequence based typing (SBT), PCR-NGS is extremely labor intensive and ti...

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Detalles Bibliográficos
Autores principales: Ozaki, Yuki, Suzuki, Shingo, Kashiwase, Koichi, Shigenari, Atsuko, Okudaira, Yuko, Ito, Sayaka, Masuya, Anri, Azuma, Fumihiro, Yabe, Toshio, Morishima, Satoko, Mitsunaga, Shigeki, Satake, Masahiro, Ota, Masao, Morishima, Yasuo, Kulski, Jerzy K, Saito, Katsuyuki, Inoko, Hidetoshi, Shiina, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404632/
https://www.ncbi.nlm.nih.gov/pubmed/25895492
http://dx.doi.org/10.1186/s12864-015-1514-4

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