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Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms
BACKGROUND: Few data are available about the inflammatory cytokine profile of bronchoalveolar lavage (BAL) from young children with frequent wheeze. The first aim was to investigate the BAL cellular and cytokine profiles in infants with recurrent lower respiratory symptoms in whom bronchoscopy was i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405820/ https://www.ncbi.nlm.nih.gov/pubmed/25905006 http://dx.doi.org/10.1186/2045-7022-4-35 |
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author | Malmström, Kristiina Lehto, Maili Majuri, Marja-Leena Paavonen, Timo Sarna, Seppo Pelkonen, Anna S Malmberg, L Pekka Lindahl, Harry Kajosaari, Merja Saglani, Sejal Alenius, Harri Mäkelä, Mika J |
author_facet | Malmström, Kristiina Lehto, Maili Majuri, Marja-Leena Paavonen, Timo Sarna, Seppo Pelkonen, Anna S Malmberg, L Pekka Lindahl, Harry Kajosaari, Merja Saglani, Sejal Alenius, Harri Mäkelä, Mika J |
author_sort | Malmström, Kristiina |
collection | PubMed |
description | BACKGROUND: Few data are available about the inflammatory cytokine profile of bronchoalveolar lavage (BAL) from young children with frequent wheeze. The first aim was to investigate the BAL cellular and cytokine profiles in infants with recurrent lower respiratory symptoms in whom bronchoscopy was indicated for clinical symptom evaluation. The second aim was to relate the BAL results with the histological findings of the endobronchial carina biopsies. METHODS: Thirty-nine infants (median age 0.9 years) underwent lung function testing by whole-body plethysmography prior to the bronchoscopy. The BAL differential cell counts and cytokine levels were quantified. These findings were compared with the histological findings of the endobronchial carina biopsies. RESULTS: The differential cytology reflected mainly that described for healthy infants with lymphocyte counts at the upper range level. A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement membrane was found. Detectable levels of pro-inflammatory cytokine proteins IL-1β, IL-17A, IL-18, IL-23, and IL-33 were found, whereas levels of Th2-type cytokine proteins were low. Frequent wheeze was the only clinical characteristic significantly related to detectable combined pro-inflammatory cytokine profile. Lung function did not correlate with any cytokine. CONCLUSIONS: A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement thickness was found. Detectable production of pro-inflammatory cytokines associated positively with frequent wheeze. |
format | Online Article Text |
id | pubmed-4405820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44058202015-04-23 Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms Malmström, Kristiina Lehto, Maili Majuri, Marja-Leena Paavonen, Timo Sarna, Seppo Pelkonen, Anna S Malmberg, L Pekka Lindahl, Harry Kajosaari, Merja Saglani, Sejal Alenius, Harri Mäkelä, Mika J Clin Transl Allergy Research BACKGROUND: Few data are available about the inflammatory cytokine profile of bronchoalveolar lavage (BAL) from young children with frequent wheeze. The first aim was to investigate the BAL cellular and cytokine profiles in infants with recurrent lower respiratory symptoms in whom bronchoscopy was indicated for clinical symptom evaluation. The second aim was to relate the BAL results with the histological findings of the endobronchial carina biopsies. METHODS: Thirty-nine infants (median age 0.9 years) underwent lung function testing by whole-body plethysmography prior to the bronchoscopy. The BAL differential cell counts and cytokine levels were quantified. These findings were compared with the histological findings of the endobronchial carina biopsies. RESULTS: The differential cytology reflected mainly that described for healthy infants with lymphocyte counts at the upper range level. A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement membrane was found. Detectable levels of pro-inflammatory cytokine proteins IL-1β, IL-17A, IL-18, IL-23, and IL-33 were found, whereas levels of Th2-type cytokine proteins were low. Frequent wheeze was the only clinical characteristic significantly related to detectable combined pro-inflammatory cytokine profile. Lung function did not correlate with any cytokine. CONCLUSIONS: A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement thickness was found. Detectable production of pro-inflammatory cytokines associated positively with frequent wheeze. BioMed Central 2014-10-24 /pmc/articles/PMC4405820/ /pubmed/25905006 http://dx.doi.org/10.1186/2045-7022-4-35 Text en © Malmström et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Malmström, Kristiina Lehto, Maili Majuri, Marja-Leena Paavonen, Timo Sarna, Seppo Pelkonen, Anna S Malmberg, L Pekka Lindahl, Harry Kajosaari, Merja Saglani, Sejal Alenius, Harri Mäkelä, Mika J Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title | Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title_full | Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title_fullStr | Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title_full_unstemmed | Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title_short | Bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
title_sort | bronchoalveolar lavage in infants with recurrent lower respiratory symptoms |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405820/ https://www.ncbi.nlm.nih.gov/pubmed/25905006 http://dx.doi.org/10.1186/2045-7022-4-35 |
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