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Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing

RNA interference (RNAi) is a mechanism of homology dependent gene silencing present in plants and animals. It operates through 21–24 nucleotides small RNAs which are processed through a set of core enzymatic machinery that involves Dicer and Argonaute proteins. In recent past, the technology has bee...

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Autores principales: Kola, Vijaya Sudhakara Rao, Renuka, P., Madhav, Maganti Sheshu, Mangrauthia, Satendra K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406143/
https://www.ncbi.nlm.nih.gov/pubmed/25954206
http://dx.doi.org/10.3389/fphys.2015.00119
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author Kola, Vijaya Sudhakara Rao
Renuka, P.
Madhav, Maganti Sheshu
Mangrauthia, Satendra K.
author_facet Kola, Vijaya Sudhakara Rao
Renuka, P.
Madhav, Maganti Sheshu
Mangrauthia, Satendra K.
author_sort Kola, Vijaya Sudhakara Rao
collection PubMed
description RNA interference (RNAi) is a mechanism of homology dependent gene silencing present in plants and animals. It operates through 21–24 nucleotides small RNAs which are processed through a set of core enzymatic machinery that involves Dicer and Argonaute proteins. In recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. The genes encoding key enzymes/proteins with the great potential for developing an effective insect control by RNAi approach are actylcholinesterase, cytochrome P450 enzymes, amino peptidase N, allatostatin, allatotropin, tryptophan oxygenase, arginine kinase, vacuolar ATPase, chitin synthase, glutathione-S-transferase, catalase, trehalose phosphate synthase, vitellogenin, hydroxy-3-methylglutaryl coenzyme A reductase, and hormone receptor genes. Through various studies, it is demonstrated that RNAi is a reliable molecular tool which offers great promises in meeting the challenges imposed by crop insects with careful selection of key enzymes/proteins. Utilization of RNAi tool to target some of these key proteins of crop insects through various approaches is described here. The major challenges of RNAi based insect control such as identifying potential targets, delivery methods of silencing trigger, off target effects, and complexity of insect biology are very well illustrated. Further, required efforts to address these challenges are also discussed.
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spelling pubmed-44061432015-05-07 Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing Kola, Vijaya Sudhakara Rao Renuka, P. Madhav, Maganti Sheshu Mangrauthia, Satendra K. Front Physiol Physiology RNA interference (RNAi) is a mechanism of homology dependent gene silencing present in plants and animals. It operates through 21–24 nucleotides small RNAs which are processed through a set of core enzymatic machinery that involves Dicer and Argonaute proteins. In recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. The genes encoding key enzymes/proteins with the great potential for developing an effective insect control by RNAi approach are actylcholinesterase, cytochrome P450 enzymes, amino peptidase N, allatostatin, allatotropin, tryptophan oxygenase, arginine kinase, vacuolar ATPase, chitin synthase, glutathione-S-transferase, catalase, trehalose phosphate synthase, vitellogenin, hydroxy-3-methylglutaryl coenzyme A reductase, and hormone receptor genes. Through various studies, it is demonstrated that RNAi is a reliable molecular tool which offers great promises in meeting the challenges imposed by crop insects with careful selection of key enzymes/proteins. Utilization of RNAi tool to target some of these key proteins of crop insects through various approaches is described here. The major challenges of RNAi based insect control such as identifying potential targets, delivery methods of silencing trigger, off target effects, and complexity of insect biology are very well illustrated. Further, required efforts to address these challenges are also discussed. Frontiers Media S.A. 2015-04-22 /pmc/articles/PMC4406143/ /pubmed/25954206 http://dx.doi.org/10.3389/fphys.2015.00119 Text en Copyright © 2015 Kola, Renuka, Madhav and Mangrauthia. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Kola, Vijaya Sudhakara Rao
Renuka, P.
Madhav, Maganti Sheshu
Mangrauthia, Satendra K.
Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title_full Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title_fullStr Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title_full_unstemmed Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title_short Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing
title_sort key enzymes and proteins of crop insects as candidate for rnai based gene silencing
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406143/
https://www.ncbi.nlm.nih.gov/pubmed/25954206
http://dx.doi.org/10.3389/fphys.2015.00119
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