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Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers

Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maint...

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Autores principales: Ji, Jiao-Jiao, Huang, Wei, Li, Zheng, Chai, Wei-Guo, Yin, Yan-Xu, Li, Da-Wei, Gong, Zhen-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406146/
https://www.ncbi.nlm.nih.gov/pubmed/25954296
http://dx.doi.org/10.3389/fpls.2015.00272
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author Ji, Jiao-Jiao
Huang, Wei
Li, Zheng
Chai, Wei-Guo
Yin, Yan-Xu
Li, Da-Wei
Gong, Zhen-Hui
author_facet Ji, Jiao-Jiao
Huang, Wei
Li, Zheng
Chai, Wei-Guo
Yin, Yan-Xu
Li, Da-Wei
Gong, Zhen-Hui
author_sort Ji, Jiao-Jiao
collection PubMed
description Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific expression of the orf507 gene in the pepper maintainer line found that transformants were morphologically similar to untransformed and transformed control plants, but had shrunken anthers that showed little dehiscence and fewer pollen grains with lower germination rate and higher naturally damaged rate. These characters were different from those of CMS line which does not produce any pollen grains. Meanwhile a pollination test using transformants as the male parent set few fruit and there were few seeds in the limited number of fruits. At the tetrad stage, ablation of the tapetal cell induced by premature programmed cell death (PCD) occurred in the transformants and the microspores were distorted and degraded at the mononuclear stage. Stable transmission of induced semi-male sterility was confirmed by a test cross. In addition, expression of orf507 in the maintainer lines seemed to inhibit expression of atp6-2 to a certain extent, and lead to the increase of the activity of cytochrome c oxidase and the ATP hydrolysis of the mitochondrial F(1)F(o)-ATP synthase. These results introduce the premature PCD caused by orf507 gene in tapetal cells and semi-male sterility, but not complete male sterility.
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spelling pubmed-44061462015-05-07 Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers Ji, Jiao-Jiao Huang, Wei Li, Zheng Chai, Wei-Guo Yin, Yan-Xu Li, Da-Wei Gong, Zhen-Hui Front Plant Sci Plant Science Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific expression of the orf507 gene in the pepper maintainer line found that transformants were morphologically similar to untransformed and transformed control plants, but had shrunken anthers that showed little dehiscence and fewer pollen grains with lower germination rate and higher naturally damaged rate. These characters were different from those of CMS line which does not produce any pollen grains. Meanwhile a pollination test using transformants as the male parent set few fruit and there were few seeds in the limited number of fruits. At the tetrad stage, ablation of the tapetal cell induced by premature programmed cell death (PCD) occurred in the transformants and the microspores were distorted and degraded at the mononuclear stage. Stable transmission of induced semi-male sterility was confirmed by a test cross. In addition, expression of orf507 in the maintainer lines seemed to inhibit expression of atp6-2 to a certain extent, and lead to the increase of the activity of cytochrome c oxidase and the ATP hydrolysis of the mitochondrial F(1)F(o)-ATP synthase. These results introduce the premature PCD caused by orf507 gene in tapetal cells and semi-male sterility, but not complete male sterility. Frontiers Media S.A. 2015-04-22 /pmc/articles/PMC4406146/ /pubmed/25954296 http://dx.doi.org/10.3389/fpls.2015.00272 Text en Copyright © 2015 Ji, Huang, Li, Chai, Yin, Li and Gong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ji, Jiao-Jiao
Huang, Wei
Li, Zheng
Chai, Wei-Guo
Yin, Yan-Xu
Li, Da-Wei
Gong, Zhen-Hui
Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_full Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_fullStr Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_full_unstemmed Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_short Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
title_sort tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406146/
https://www.ncbi.nlm.nih.gov/pubmed/25954296
http://dx.doi.org/10.3389/fpls.2015.00272
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