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Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals wer...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406835/ https://www.ncbi.nlm.nih.gov/pubmed/25909042 http://dx.doi.org/10.3732/apps.1400120 |
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author | Bozzi, Jorge A. Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo A. Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
author_facet | Bozzi, Jorge A. Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo A. Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
author_sort | Bozzi, Jorge A. |
collection | PubMed |
description | PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. CONCLUSIONS: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. |
format | Online Article Text |
id | pubmed-4406835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-44068352015-04-23 Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) Bozzi, Jorge A. Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo A. Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina Appl Plant Sci Primer Note PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. CONCLUSIONS: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. Botanical Society of America 2015-04-07 /pmc/articles/PMC4406835/ /pubmed/25909042 http://dx.doi.org/10.3732/apps.1400120 Text en © 2015 Bozzi et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Bozzi, Jorge A. Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo A. Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title_full | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title_fullStr | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title_full_unstemmed | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title_short | Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
title_sort | characterization of 23 polymorphic ssr markers in salix humboldtiana (salicaceae) using next-generation sequencing and cross-amplification from related species(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406835/ https://www.ncbi.nlm.nih.gov/pubmed/25909042 http://dx.doi.org/10.3732/apps.1400120 |
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