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Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)

PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals wer...

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Autores principales: Bozzi, Jorge A., Liepelt, Sascha, Ohneiser, Sebastian, Gallo, Leonardo A., Marchelli, Paula, Leyer, Ilona, Ziegenhagen, Birgit, Mengel, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406835/
https://www.ncbi.nlm.nih.gov/pubmed/25909042
http://dx.doi.org/10.3732/apps.1400120
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author Bozzi, Jorge A.
Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo A.
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author_facet Bozzi, Jorge A.
Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo A.
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author_sort Bozzi, Jorge A.
collection PubMed
description PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. CONCLUSIONS: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
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spelling pubmed-44068352015-04-23 Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1) Bozzi, Jorge A. Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo A. Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina Appl Plant Sci Primer Note PREMISE OF THE STUDY: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. CONCLUSIONS: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. Botanical Society of America 2015-04-07 /pmc/articles/PMC4406835/ /pubmed/25909042 http://dx.doi.org/10.3732/apps.1400120 Text en © 2015 Bozzi et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Bozzi, Jorge A.
Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo A.
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title_full Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title_fullStr Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title_full_unstemmed Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title_short Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species(1)
title_sort characterization of 23 polymorphic ssr markers in salix humboldtiana (salicaceae) using next-generation sequencing and cross-amplification from related species(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406835/
https://www.ncbi.nlm.nih.gov/pubmed/25909042
http://dx.doi.org/10.3732/apps.1400120
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