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Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1)
PREMISE OF THE STUDY: We developed microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae. METHODS AND RESULTS: We sequenced a small insert ge...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406837/ https://www.ncbi.nlm.nih.gov/pubmed/25909044 http://dx.doi.org/10.3732/apps.1400123 |
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author | Hatmaker, E. Anne Wadl, Phillip A. Mantooth, Kristie Scheffler, Brian E. Ownley, Bonnie H. Trigiano, Robert N. |
author_facet | Hatmaker, E. Anne Wadl, Phillip A. Mantooth, Kristie Scheffler, Brian E. Ownley, Bonnie H. Trigiano, Robert N. |
author_sort | Hatmaker, E. Anne |
collection | PubMed |
description | PREMISE OF THE STUDY: We developed microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae. METHODS AND RESULTS: We sequenced a small insert genomic library enriched for microsatellites to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from four to 15 across five genera within Hamamelidaceae. Shannon’s information index ranged from 0.07 to 0.14. CONCLUSIONS: These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for genetic studies of five popular genera of woody ornamental plants. |
format | Online Article Text |
id | pubmed-4406837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-44068372015-04-23 Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) Hatmaker, E. Anne Wadl, Phillip A. Mantooth, Kristie Scheffler, Brian E. Ownley, Bonnie H. Trigiano, Robert N. Appl Plant Sci Primer Note PREMISE OF THE STUDY: We developed microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae. METHODS AND RESULTS: We sequenced a small insert genomic library enriched for microsatellites to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from four to 15 across five genera within Hamamelidaceae. Shannon’s information index ranged from 0.07 to 0.14. CONCLUSIONS: These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for genetic studies of five popular genera of woody ornamental plants. Botanical Society of America 2015-04-07 /pmc/articles/PMC4406837/ /pubmed/25909044 http://dx.doi.org/10.3732/apps.1400123 Text en © 2015 Hatmaker et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This article is a U.S. Government work and is in the public domain in the USA. |
spellingShingle | Primer Note Hatmaker, E. Anne Wadl, Phillip A. Mantooth, Kristie Scheffler, Brian E. Ownley, Bonnie H. Trigiano, Robert N. Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title | Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title_full | Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title_fullStr | Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title_full_unstemmed | Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title_short | Development of microsatellites from Fothergilla ×intermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae(1) |
title_sort | development of microsatellites from fothergilla ×intermedia (hamamelidaceae) and cross transfer to four other genera within hamamelidaceae(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406837/ https://www.ncbi.nlm.nih.gov/pubmed/25909044 http://dx.doi.org/10.3732/apps.1400123 |
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