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Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans

Mycobacterium ulcerans, the etiologic agent of Buruli ulcer, has been detected on aquatic plants in endemic tropical regions. Here, we tested the effect of several tropical plant extracts on the growth of M. ulcerans and the closely related Mycobacterium marinum. M. ulcerans and M. marinum were inoc...

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Autores principales: Mougin, Benjamin, Tian, Roger B. D., Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408112/
https://www.ncbi.nlm.nih.gov/pubmed/25905816
http://dx.doi.org/10.1371/journal.pone.0124626
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author Mougin, Benjamin
Tian, Roger B. D.
Drancourt, Michel
author_facet Mougin, Benjamin
Tian, Roger B. D.
Drancourt, Michel
author_sort Mougin, Benjamin
collection PubMed
description Mycobacterium ulcerans, the etiologic agent of Buruli ulcer, has been detected on aquatic plants in endemic tropical regions. Here, we tested the effect of several tropical plant extracts on the growth of M. ulcerans and the closely related Mycobacterium marinum. M. ulcerans and M. marinum were inoculated on Middlebrook 7H11 medium with and without extracts from tropical aquatic plants, including Ammannia gracilis, Crinum calamistratum, Echinodorus africanus, Vallisneria nana and Vallisneria torta. Delay of detection of the first colony and the number of colonies at day 7 (M. marinum) or day 16 (M. ulcerans) were used as endpoints. The first M. ulcerans colonies were detected at 8 ± 0 days on control Middlebrook 7H11 medium, 6.34 ± 0.75 days on A. gracilis-enriched medium (p<0.01), 6 ± 1 days on E. africanus- and V. torta-enriched media (p<0.01), 6 ± 0 days on V. nana-enriched medium (p<0.01) and 5.67 ± 0.47 days on C. calamistratum-enriched medium (p<0.01). Furthermore, the number of detected colonies was significantly increased in C. calamistratum- and E. africanus-enriched media at each time point compared to Middlebrook 7H11 (p<0.05). V. nana- and V. torta-enriched media significantly increased the number of detected colonies starting from day 6 and day 10, respectively (p<0.001). At the opposite, A. gracilis-enriched medium significantly decreased the number of detected colonies starting from day 8 PI (p<0.05). In conclusion, some aquatic plant extracts, could be added as adjuvants to the Middlebrook 7H11 medium for the culturing of M. marinum and M. ulcerans.
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spelling pubmed-44081122015-05-04 Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans Mougin, Benjamin Tian, Roger B. D. Drancourt, Michel PLoS One Research Article Mycobacterium ulcerans, the etiologic agent of Buruli ulcer, has been detected on aquatic plants in endemic tropical regions. Here, we tested the effect of several tropical plant extracts on the growth of M. ulcerans and the closely related Mycobacterium marinum. M. ulcerans and M. marinum were inoculated on Middlebrook 7H11 medium with and without extracts from tropical aquatic plants, including Ammannia gracilis, Crinum calamistratum, Echinodorus africanus, Vallisneria nana and Vallisneria torta. Delay of detection of the first colony and the number of colonies at day 7 (M. marinum) or day 16 (M. ulcerans) were used as endpoints. The first M. ulcerans colonies were detected at 8 ± 0 days on control Middlebrook 7H11 medium, 6.34 ± 0.75 days on A. gracilis-enriched medium (p<0.01), 6 ± 1 days on E. africanus- and V. torta-enriched media (p<0.01), 6 ± 0 days on V. nana-enriched medium (p<0.01) and 5.67 ± 0.47 days on C. calamistratum-enriched medium (p<0.01). Furthermore, the number of detected colonies was significantly increased in C. calamistratum- and E. africanus-enriched media at each time point compared to Middlebrook 7H11 (p<0.05). V. nana- and V. torta-enriched media significantly increased the number of detected colonies starting from day 6 and day 10, respectively (p<0.001). At the opposite, A. gracilis-enriched medium significantly decreased the number of detected colonies starting from day 8 PI (p<0.05). In conclusion, some aquatic plant extracts, could be added as adjuvants to the Middlebrook 7H11 medium for the culturing of M. marinum and M. ulcerans. Public Library of Science 2015-04-23 /pmc/articles/PMC4408112/ /pubmed/25905816 http://dx.doi.org/10.1371/journal.pone.0124626 Text en © 2015 Mougin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mougin, Benjamin
Tian, Roger B. D.
Drancourt, Michel
Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title_full Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title_fullStr Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title_full_unstemmed Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title_short Tropical Plant Extracts Modulating the Growth of Mycobacterium ulcerans
title_sort tropical plant extracts modulating the growth of mycobacterium ulcerans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408112/
https://www.ncbi.nlm.nih.gov/pubmed/25905816
http://dx.doi.org/10.1371/journal.pone.0124626
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