A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis

We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity...

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Detalles Bibliográficos
Autores principales: Hamasur, Beston, Bruchfeld, Judith, van Helden, Paul, Källenius, Gunilla, Svenson, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408114/
https://www.ncbi.nlm.nih.gov/pubmed/25905641
http://dx.doi.org/10.1371/journal.pone.0123457
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author Hamasur, Beston
Bruchfeld, Judith
van Helden, Paul
Källenius, Gunilla
Svenson, Stefan
author_facet Hamasur, Beston
Bruchfeld, Judith
van Helden, Paul
Källenius, Gunilla
Svenson, Stefan
author_sort Hamasur, Beston
collection PubMed
description We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIV-negative TB patients was very low.
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spelling pubmed-44081142015-05-04 A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis Hamasur, Beston Bruchfeld, Judith van Helden, Paul Källenius, Gunilla Svenson, Stefan PLoS One Research Article We have previously developed a diagnostic test for tuberculosis based on detection of mycobacterial lipoarabinomannan (LAM) in urine. The method depended on a laborious concentration step. We have now developed an easy to perform test based on a magnetic immunoassay platform, utilizing high avidity monoclonal antibodies for the detection of LAM in urine. With this method the analytical sensitivity of the assay was increased 50-100-fold compared to conventional ELISA. In a pilot study of HIV-negative patients with microbiologically verified TB (n=17) and healthy controls (n=22) the sensitivity of the test was 82% and the specificity 100%. This is in stark positive contrast to a range of studies using available commercial tests with polyclonal anti-LAM Abs where the sensitivity of the tests in HIV-negative TB patients was very low. Public Library of Science 2015-04-23 /pmc/articles/PMC4408114/ /pubmed/25905641 http://dx.doi.org/10.1371/journal.pone.0123457 Text en © 2015 Hamasur et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hamasur, Beston
Bruchfeld, Judith
van Helden, Paul
Källenius, Gunilla
Svenson, Stefan
A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title_full A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title_fullStr A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title_full_unstemmed A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title_short A Sensitive Urinary Lipoarabinomannan Test for Tuberculosis
title_sort sensitive urinary lipoarabinomannan test for tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408114/
https://www.ncbi.nlm.nih.gov/pubmed/25905641
http://dx.doi.org/10.1371/journal.pone.0123457
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