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Genetic characterization of caffeine degradation by bacteria and its potential applications
The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408171/ https://www.ncbi.nlm.nih.gov/pubmed/25678373 http://dx.doi.org/10.1111/1751-7915.12262 |
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author | Summers, Ryan M Mohanty, Sujit K Gopishetty, Sridhar Subramanian, Mani |
author_facet | Summers, Ryan M Mohanty, Sujit K Gopishetty, Sridhar Subramanian, Mani |
author_sort | Summers, Ryan M |
collection | PubMed |
description | The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N-demethylation and C-8 oxidation. All of the genes for the N-demethylation pathway, encoding enzymes in the Rieske oxygenase family, reside on 13.2-kb genomic DNA fragment found in Pseudomonas putida CBB5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in Pseudomonas sp. CES. Similarly, genes for C-8 oxidation of caffeine have been located on a 25.2-kb genomic DNA fragment of Pseudomonas sp. CBB1. The C-8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of Klebsiella pneumoniae. Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio-decaffeination, remediation of caffeine-contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated. |
format | Online Article Text |
id | pubmed-4408171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44081712015-05-01 Genetic characterization of caffeine degradation by bacteria and its potential applications Summers, Ryan M Mohanty, Sujit K Gopishetty, Sridhar Subramanian, Mani Microb Biotechnol Minireview The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N-demethylation and C-8 oxidation. All of the genes for the N-demethylation pathway, encoding enzymes in the Rieske oxygenase family, reside on 13.2-kb genomic DNA fragment found in Pseudomonas putida CBB5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in Pseudomonas sp. CES. Similarly, genes for C-8 oxidation of caffeine have been located on a 25.2-kb genomic DNA fragment of Pseudomonas sp. CBB1. The C-8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of Klebsiella pneumoniae. Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio-decaffeination, remediation of caffeine-contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated. BlackWell Publishing Ltd 2015-05 2015-02-12 /pmc/articles/PMC4408171/ /pubmed/25678373 http://dx.doi.org/10.1111/1751-7915.12262 Text en © 2015 The Author. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Minireview Summers, Ryan M Mohanty, Sujit K Gopishetty, Sridhar Subramanian, Mani Genetic characterization of caffeine degradation by bacteria and its potential applications |
title | Genetic characterization of caffeine degradation by bacteria and its potential applications |
title_full | Genetic characterization of caffeine degradation by bacteria and its potential applications |
title_fullStr | Genetic characterization of caffeine degradation by bacteria and its potential applications |
title_full_unstemmed | Genetic characterization of caffeine degradation by bacteria and its potential applications |
title_short | Genetic characterization of caffeine degradation by bacteria and its potential applications |
title_sort | genetic characterization of caffeine degradation by bacteria and its potential applications |
topic | Minireview |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408171/ https://www.ncbi.nlm.nih.gov/pubmed/25678373 http://dx.doi.org/10.1111/1751-7915.12262 |
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