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Genetic characterization of caffeine degradation by bacteria and its potential applications

The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basi...

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Autores principales: Summers, Ryan M, Mohanty, Sujit K, Gopishetty, Sridhar, Subramanian, Mani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408171/
https://www.ncbi.nlm.nih.gov/pubmed/25678373
http://dx.doi.org/10.1111/1751-7915.12262
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author Summers, Ryan M
Mohanty, Sujit K
Gopishetty, Sridhar
Subramanian, Mani
author_facet Summers, Ryan M
Mohanty, Sujit K
Gopishetty, Sridhar
Subramanian, Mani
author_sort Summers, Ryan M
collection PubMed
description The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N-demethylation and C-8 oxidation. All of the genes for the N-demethylation pathway, encoding enzymes in the Rieske oxygenase family, reside on 13.2-kb genomic DNA fragment found in Pseudomonas putida CBB5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in Pseudomonas sp. CES. Similarly, genes for C-8 oxidation of caffeine have been located on a 25.2-kb genomic DNA fragment of Pseudomonas sp. CBB1. The C-8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of Klebsiella pneumoniae. Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio-decaffeination, remediation of caffeine-contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated.
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spelling pubmed-44081712015-05-01 Genetic characterization of caffeine degradation by bacteria and its potential applications Summers, Ryan M Mohanty, Sujit K Gopishetty, Sridhar Subramanian, Mani Microb Biotechnol Minireview The ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N-demethylation and C-8 oxidation. All of the genes for the N-demethylation pathway, encoding enzymes in the Rieske oxygenase family, reside on 13.2-kb genomic DNA fragment found in Pseudomonas putida CBB5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in Pseudomonas sp. CES. Similarly, genes for C-8 oxidation of caffeine have been located on a 25.2-kb genomic DNA fragment of Pseudomonas sp. CBB1. The C-8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of Klebsiella pneumoniae. Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio-decaffeination, remediation of caffeine-contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated. BlackWell Publishing Ltd 2015-05 2015-02-12 /pmc/articles/PMC4408171/ /pubmed/25678373 http://dx.doi.org/10.1111/1751-7915.12262 Text en © 2015 The Author. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireview
Summers, Ryan M
Mohanty, Sujit K
Gopishetty, Sridhar
Subramanian, Mani
Genetic characterization of caffeine degradation by bacteria and its potential applications
title Genetic characterization of caffeine degradation by bacteria and its potential applications
title_full Genetic characterization of caffeine degradation by bacteria and its potential applications
title_fullStr Genetic characterization of caffeine degradation by bacteria and its potential applications
title_full_unstemmed Genetic characterization of caffeine degradation by bacteria and its potential applications
title_short Genetic characterization of caffeine degradation by bacteria and its potential applications
title_sort genetic characterization of caffeine degradation by bacteria and its potential applications
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408171/
https://www.ncbi.nlm.nih.gov/pubmed/25678373
http://dx.doi.org/10.1111/1751-7915.12262
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