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The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin
When we expressed a small (0.9 kb) nonprotein-coding transcript derived from the mitochondrial VS plasmid in the nucleus of Neurospora we found that it was efficiently spliced at one or more of eight 5′ splice sites and ten 3′ splice sites, which are present apparently by chance in the sequence. Fur...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408805/ https://www.ncbi.nlm.nih.gov/pubmed/25805857 http://dx.doi.org/10.1261/rna.047985.114 |
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author | Collins, Richard A. Stajich, Jason E. Field, Deborah J. Olive, Joan E. DeAbreu, Diane M. |
author_facet | Collins, Richard A. Stajich, Jason E. Field, Deborah J. Olive, Joan E. DeAbreu, Diane M. |
author_sort | Collins, Richard A. |
collection | PubMed |
description | When we expressed a small (0.9 kb) nonprotein-coding transcript derived from the mitochondrial VS plasmid in the nucleus of Neurospora we found that it was efficiently spliced at one or more of eight 5′ splice sites and ten 3′ splice sites, which are present apparently by chance in the sequence. Further experimental and bioinformatic analyses of other mitochondrial plasmids, random sequences, and natural nuclear genes in Neurospora and other fungi indicate that fungal spliceosomes recognize a wide range of 5′ splice site and branchpoint sequences and predict introns to be present at high frequency in random sequence. In contrast, analysis of intronless fungal nuclear genes indicates that branchpoint, 5′ splice site and 3′ splice site consensus sequences are underrepresented compared with random sequences. This underrepresentation of splicing signals is sufficient to deplete the nuclear genome of splice sites at locations that do not comprise biologically relevant introns. Thus, the splicing machinery can recognize a wide range of splicing signal sequences, but splicing still occurs with great accuracy, not because the splicing machinery distinguishes correct from incorrect introns, but because incorrect introns are substantially depleted from the genome. |
format | Online Article Text |
id | pubmed-4408805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44088052016-05-01 The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin Collins, Richard A. Stajich, Jason E. Field, Deborah J. Olive, Joan E. DeAbreu, Diane M. RNA Articles When we expressed a small (0.9 kb) nonprotein-coding transcript derived from the mitochondrial VS plasmid in the nucleus of Neurospora we found that it was efficiently spliced at one or more of eight 5′ splice sites and ten 3′ splice sites, which are present apparently by chance in the sequence. Further experimental and bioinformatic analyses of other mitochondrial plasmids, random sequences, and natural nuclear genes in Neurospora and other fungi indicate that fungal spliceosomes recognize a wide range of 5′ splice site and branchpoint sequences and predict introns to be present at high frequency in random sequence. In contrast, analysis of intronless fungal nuclear genes indicates that branchpoint, 5′ splice site and 3′ splice site consensus sequences are underrepresented compared with random sequences. This underrepresentation of splicing signals is sufficient to deplete the nuclear genome of splice sites at locations that do not comprise biologically relevant introns. Thus, the splicing machinery can recognize a wide range of splicing signal sequences, but splicing still occurs with great accuracy, not because the splicing machinery distinguishes correct from incorrect introns, but because incorrect introns are substantially depleted from the genome. Cold Spring Harbor Laboratory Press 2015-05 /pmc/articles/PMC4408805/ /pubmed/25805857 http://dx.doi.org/10.1261/rna.047985.114 Text en © 2015 Collins et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Articles Collins, Richard A. Stajich, Jason E. Field, Deborah J. Olive, Joan E. DeAbreu, Diane M. The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title | The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title_full | The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title_fullStr | The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title_full_unstemmed | The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title_short | The low information content of Neurospora splicing signals: implications for RNA splicing and intron origin |
title_sort | low information content of neurospora splicing signals: implications for rna splicing and intron origin |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408805/ https://www.ncbi.nlm.nih.gov/pubmed/25805857 http://dx.doi.org/10.1261/rna.047985.114 |
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