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Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion

PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY:...

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Autores principales: Poulton, Katie, Ripoll, Vera M, Pericleous, Charis, Meroni, Pier Luigi, Gerosa, Maria, Ioannou, Yiannis, Rahman, Anisur, Giles, Ian P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409084/
https://www.ncbi.nlm.nih.gov/pubmed/25469631
http://dx.doi.org/10.1111/aji.12341
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author Poulton, Katie
Ripoll, Vera M
Pericleous, Charis
Meroni, Pier Luigi
Gerosa, Maria
Ioannou, Yiannis
Rahman, Anisur
Giles, Ian P
author_facet Poulton, Katie
Ripoll, Vera M
Pericleous, Charis
Meroni, Pier Luigi
Gerosa, Maria
Ioannou, Yiannis
Rahman, Anisur
Giles, Ian P
author_sort Poulton, Katie
collection PubMed
description PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY: HTR-8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. RESULTS: VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. CONCLUSIONS: VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not.
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spelling pubmed-44090842015-04-29 Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion Poulton, Katie Ripoll, Vera M Pericleous, Charis Meroni, Pier Luigi Gerosa, Maria Ioannou, Yiannis Rahman, Anisur Giles, Ian P Am J Reprod Immunol Immunological Factors in Pregnancy PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY: HTR-8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. RESULTS: VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. CONCLUSIONS: VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not. BlackWell Publishing Ltd 2015-05 2014-12-02 /pmc/articles/PMC4409084/ /pubmed/25469631 http://dx.doi.org/10.1111/aji.12341 Text en © 2014 The Authors American Journal of Reproductive Immunology Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Immunological Factors in Pregnancy
Poulton, Katie
Ripoll, Vera M
Pericleous, Charis
Meroni, Pier Luigi
Gerosa, Maria
Ioannou, Yiannis
Rahman, Anisur
Giles, Ian P
Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title_full Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title_fullStr Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title_full_unstemmed Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title_short Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
title_sort purified igg from patients with obstetric but not igg from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion
topic Immunological Factors in Pregnancy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409084/
https://www.ncbi.nlm.nih.gov/pubmed/25469631
http://dx.doi.org/10.1111/aji.12341
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