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Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion
PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY:...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409084/ https://www.ncbi.nlm.nih.gov/pubmed/25469631 http://dx.doi.org/10.1111/aji.12341 |
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author | Poulton, Katie Ripoll, Vera M Pericleous, Charis Meroni, Pier Luigi Gerosa, Maria Ioannou, Yiannis Rahman, Anisur Giles, Ian P |
author_facet | Poulton, Katie Ripoll, Vera M Pericleous, Charis Meroni, Pier Luigi Gerosa, Maria Ioannou, Yiannis Rahman, Anisur Giles, Ian P |
author_sort | Poulton, Katie |
collection | PubMed |
description | PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY: HTR-8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. RESULTS: VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. CONCLUSIONS: VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not. |
format | Online Article Text |
id | pubmed-4409084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44090842015-04-29 Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion Poulton, Katie Ripoll, Vera M Pericleous, Charis Meroni, Pier Luigi Gerosa, Maria Ioannou, Yiannis Rahman, Anisur Giles, Ian P Am J Reprod Immunol Immunological Factors in Pregnancy PROBLEM: Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first-trimester trophoblast (HTR8) cells. METHOD OF STUDY: HTR-8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6. RESULTS: VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. CONCLUSIONS: VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not. BlackWell Publishing Ltd 2015-05 2014-12-02 /pmc/articles/PMC4409084/ /pubmed/25469631 http://dx.doi.org/10.1111/aji.12341 Text en © 2014 The Authors American Journal of Reproductive Immunology Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Immunological Factors in Pregnancy Poulton, Katie Ripoll, Vera M Pericleous, Charis Meroni, Pier Luigi Gerosa, Maria Ioannou, Yiannis Rahman, Anisur Giles, Ian P Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title | Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title_full | Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title_fullStr | Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title_full_unstemmed | Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title_short | Purified IgG from Patients with Obstetric but not IgG from Non-obstetric Antiphospholipid Syndrome Inhibit Trophoblast Invasion |
title_sort | purified igg from patients with obstetric but not igg from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion |
topic | Immunological Factors in Pregnancy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409084/ https://www.ncbi.nlm.nih.gov/pubmed/25469631 http://dx.doi.org/10.1111/aji.12341 |
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