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Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds
In a previous study about low-level laser therapy biomodulation on a full-thickness burn model we showed that single and fractionated dose regimens increased wound healing and leukocyte influx similarly when compared with untreated control. In order to verify if this finding would be similar in an i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409316/ https://www.ncbi.nlm.nih.gov/pubmed/25909480 http://dx.doi.org/10.1371/journal.pone.0122042 |
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author | de Loura Santana, Cristiano de Fátima Teixeira Silva, Daniela Deana, Alessandro Melo Prates, Renato Araujo Souza, Amanda Pires Gomes, Mariana Teixeira de Azevedo Sampaio, Brunna Pileggi Shibuya, Josiane Ferraretto Bussadori, Sandra Kalil Mesquita-Ferrari, Raquel Agnelli Fernandes, Kristianne Porta Santos França, Cristiane Miranda |
author_facet | de Loura Santana, Cristiano de Fátima Teixeira Silva, Daniela Deana, Alessandro Melo Prates, Renato Araujo Souza, Amanda Pires Gomes, Mariana Teixeira de Azevedo Sampaio, Brunna Pileggi Shibuya, Josiane Ferraretto Bussadori, Sandra Kalil Mesquita-Ferrari, Raquel Agnelli Fernandes, Kristianne Porta Santos França, Cristiane Miranda |
author_sort | de Loura Santana, Cristiano |
collection | PubMed |
description | In a previous study about low-level laser therapy biomodulation on a full-thickness burn model we showed that single and fractionated dose regimens increased wound healing and leukocyte influx similarly when compared with untreated control. In order to verify if this finding would be similar in an impaired wound model, we investigated the effect of single and multiple irradiations on wound closure rate, type of inflammatory infiltrate, myofibroblasts, collagen deposition, and optical retardation of collagen in diabetic rats. Female Wistar rats in the same estrous cycle had diabetes induced with streptozotocin and an 8-mm excisional wound performed with a punch. The experimental groups were: control group – untreated ulcer; single-dose group – ulcer submitted to single dose of diode laser therapy (λ = 660 ± 2 nm; P = 30 mW; energy density: 4 J/cm(2)) and fractionated-dose group – ulcer submitted to 1 J/cm(2) laser therapy on Days 1, 3, 8, and 10. The ulcers were photographed on the experimental days and after euthanasia tissue samples were routinely processed for histological and immunohistochemistry analyses. Independently of the energy density, laser therapy accelerated wound closure by approximately 40% in the first three days in comparison to the control group. Laser therapy increased acute inflammatory infiltrate until Day 3. Both laser groups exhibited more myofibroblasts and better collagen organization than the control group. The findings demonstrate that low-level laser therapy in the immediate postoperative period can enhance the tissue repair process in a diabetes model. Similar effects were achieved with laser therapy applied a single time with an energy density of 4 J/cm(2) and applied four times with an energy density of 1 J/cm(2). The application of laser therapy in the inflammatory phase was the most important factor to the enhancement of the tissue repair process. |
format | Online Article Text |
id | pubmed-4409316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44093162015-05-12 Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds de Loura Santana, Cristiano de Fátima Teixeira Silva, Daniela Deana, Alessandro Melo Prates, Renato Araujo Souza, Amanda Pires Gomes, Mariana Teixeira de Azevedo Sampaio, Brunna Pileggi Shibuya, Josiane Ferraretto Bussadori, Sandra Kalil Mesquita-Ferrari, Raquel Agnelli Fernandes, Kristianne Porta Santos França, Cristiane Miranda PLoS One Research Article In a previous study about low-level laser therapy biomodulation on a full-thickness burn model we showed that single and fractionated dose regimens increased wound healing and leukocyte influx similarly when compared with untreated control. In order to verify if this finding would be similar in an impaired wound model, we investigated the effect of single and multiple irradiations on wound closure rate, type of inflammatory infiltrate, myofibroblasts, collagen deposition, and optical retardation of collagen in diabetic rats. Female Wistar rats in the same estrous cycle had diabetes induced with streptozotocin and an 8-mm excisional wound performed with a punch. The experimental groups were: control group – untreated ulcer; single-dose group – ulcer submitted to single dose of diode laser therapy (λ = 660 ± 2 nm; P = 30 mW; energy density: 4 J/cm(2)) and fractionated-dose group – ulcer submitted to 1 J/cm(2) laser therapy on Days 1, 3, 8, and 10. The ulcers were photographed on the experimental days and after euthanasia tissue samples were routinely processed for histological and immunohistochemistry analyses. Independently of the energy density, laser therapy accelerated wound closure by approximately 40% in the first three days in comparison to the control group. Laser therapy increased acute inflammatory infiltrate until Day 3. Both laser groups exhibited more myofibroblasts and better collagen organization than the control group. The findings demonstrate that low-level laser therapy in the immediate postoperative period can enhance the tissue repair process in a diabetes model. Similar effects were achieved with laser therapy applied a single time with an energy density of 4 J/cm(2) and applied four times with an energy density of 1 J/cm(2). The application of laser therapy in the inflammatory phase was the most important factor to the enhancement of the tissue repair process. Public Library of Science 2015-04-24 /pmc/articles/PMC4409316/ /pubmed/25909480 http://dx.doi.org/10.1371/journal.pone.0122042 Text en © 2015 de Loura Santana et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article de Loura Santana, Cristiano de Fátima Teixeira Silva, Daniela Deana, Alessandro Melo Prates, Renato Araujo Souza, Amanda Pires Gomes, Mariana Teixeira de Azevedo Sampaio, Brunna Pileggi Shibuya, Josiane Ferraretto Bussadori, Sandra Kalil Mesquita-Ferrari, Raquel Agnelli Fernandes, Kristianne Porta Santos França, Cristiane Miranda Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title | Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title_full | Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title_fullStr | Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title_full_unstemmed | Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title_short | Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds |
title_sort | tissue responses to postoperative laser therapy in diabetic rats submitted to excisional wounds |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409316/ https://www.ncbi.nlm.nih.gov/pubmed/25909480 http://dx.doi.org/10.1371/journal.pone.0122042 |
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