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Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer
We evaluated the influence of the cancer microenvironment formed by peritoneal invasion (CMPI) on clinical findings in colon cancer patients. In addition to the association with poor prognosis, we discovered a relationship with bowel obstruction. Detailed analysis revealed that clinical findings rel...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409892/ https://www.ncbi.nlm.nih.gov/pubmed/25613547 http://dx.doi.org/10.1111/cas.12615 |
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author | Yokota, Mitsuru Kojima, Motohiro Higuchi, Youichi Nishizawa, Yuji Kobayashi, Akihiro Ito, Masaaki Saito, Norio Ochiai, Atsushi |
author_facet | Yokota, Mitsuru Kojima, Motohiro Higuchi, Youichi Nishizawa, Yuji Kobayashi, Akihiro Ito, Masaaki Saito, Norio Ochiai, Atsushi |
author_sort | Yokota, Mitsuru |
collection | PubMed |
description | We evaluated the influence of the cancer microenvironment formed by peritoneal invasion (CMPI) on clinical findings in colon cancer patients. In addition to the association with poor prognosis, we discovered a relationship with bowel obstruction. Detailed analysis revealed that clinical findings related to bowel obstruction occurred more frequently in patients with an elevated type tumor, which had peritoneal elastic laminal elevation to the tumor surface, compared to those with non-elevated type tumors among those with elastic laminal invasion (ELI). Lateral tumor spread and increase of tumor annularity rate in ELI-positive elevated type cases suggested the morphological progression from ELI-positive non-elevated type to elevated type. In addition, α-smooth muscle actin expression was the highest in ELI-positive elevated type, and prominent expressions were found not only in the deep tumor area but also in the shallow tumor area. Furthermore, contraction assays revealed the robust contractile ability of subperitoneal fibroblasts stimulated by cancer cell-conditioned medium. Our findings suggest that CMPI spread into the luminal side of the colonic wall along with tumor progression, which caused bowel obstruction through the activation of subperitoneal fibroblasts. However, although the clinical outcome was not different between the two types, the clinical findings were affected by the spread of CMPI. We are the first to explore how the alteration of the tumor-promoting microenvironment, along with tumor progression, contributes to the development of clinical findings. |
format | Online Article Text |
id | pubmed-4409892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44098922015-10-05 Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer Yokota, Mitsuru Kojima, Motohiro Higuchi, Youichi Nishizawa, Yuji Kobayashi, Akihiro Ito, Masaaki Saito, Norio Ochiai, Atsushi Cancer Sci Original Articles We evaluated the influence of the cancer microenvironment formed by peritoneal invasion (CMPI) on clinical findings in colon cancer patients. In addition to the association with poor prognosis, we discovered a relationship with bowel obstruction. Detailed analysis revealed that clinical findings related to bowel obstruction occurred more frequently in patients with an elevated type tumor, which had peritoneal elastic laminal elevation to the tumor surface, compared to those with non-elevated type tumors among those with elastic laminal invasion (ELI). Lateral tumor spread and increase of tumor annularity rate in ELI-positive elevated type cases suggested the morphological progression from ELI-positive non-elevated type to elevated type. In addition, α-smooth muscle actin expression was the highest in ELI-positive elevated type, and prominent expressions were found not only in the deep tumor area but also in the shallow tumor area. Furthermore, contraction assays revealed the robust contractile ability of subperitoneal fibroblasts stimulated by cancer cell-conditioned medium. Our findings suggest that CMPI spread into the luminal side of the colonic wall along with tumor progression, which caused bowel obstruction through the activation of subperitoneal fibroblasts. However, although the clinical outcome was not different between the two types, the clinical findings were affected by the spread of CMPI. We are the first to explore how the alteration of the tumor-promoting microenvironment, along with tumor progression, contributes to the development of clinical findings. BlackWell Publishing Ltd 2015-04 2015-03-19 /pmc/articles/PMC4409892/ /pubmed/25613547 http://dx.doi.org/10.1111/cas.12615 Text en © 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Yokota, Mitsuru Kojima, Motohiro Higuchi, Youichi Nishizawa, Yuji Kobayashi, Akihiro Ito, Masaaki Saito, Norio Ochiai, Atsushi Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title | Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title_full | Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title_fullStr | Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title_full_unstemmed | Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title_short | Spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
title_sort | spread of tumor microenvironment contributes to colonic obstruction through subperitoneal fibroblast activation in colon cancer |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409892/ https://www.ncbi.nlm.nih.gov/pubmed/25613547 http://dx.doi.org/10.1111/cas.12615 |
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