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The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice

BACKGROUND: In fertility studies, it has been shown that transforming growth factor β (TGFβ) and interlukin 10 (IL-10) play very important roles in implantation, maternal immune tolerance, placentation and fetal development, and the release beginning of release for fetal and postnatal death. The pre...

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Autores principales: Risvanli, Ali, Godekmerdan, Ahmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410039/
https://www.ncbi.nlm.nih.gov/pubmed/25918594
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author Risvanli, Ali
Godekmerdan, Ahmet
author_facet Risvanli, Ali
Godekmerdan, Ahmet
author_sort Risvanli, Ali
collection PubMed
description BACKGROUND: In fertility studies, it has been shown that transforming growth factor β (TGFβ) and interlukin 10 (IL-10) play very important roles in implantation, maternal immune tolerance, placentation and fetal development, and the release beginning of release for fetal and postnatal death. The present study aims to determine the effects of the postmating administration of neutralizing antibodies against IL-10 and TGFβ, which significantly impact pregnancy in females and the conception rates in mice via assessments of blood serum and uterine fluid concentrations of IL-2, IL-4, IL-6, IL-10, IL-17, interferon γ (IFNγ), Tumor necrosis factor α (TNFα), and TGFβ. MATERIALS AND METHODS: In this experimental study, 21 BALB/c strain female mice were mated and randomly divided into three groups. The mice in the first group were selected as the control group. The second group of animals was injected with 0.5 mg of anti-IL-10 after mating, while those in the third group were intraperitoneally injected with 0.5 mg of anti-TGFβ. The animals in all groups were decapitated on the 13thday after mating and their blood samples were taken. The uteri were removed to determine pregnancy. The mice’s uterine irrigation fluids were also obtained. We used the multiplex immunoassay technique to determine the cytokine concentrations in uterine fluid and blood serum of the mice. RESULTS: We observed no intergroup difference with respect to conception rates. A comparison of the cytokine concentrations in the uterine fluids of pregnant mice revealed higher TGFβ concentrations (p<0.01) in the second group injected with the anti-IL-10 antibody compared with the other groups. There was no difference detected in pregnant animals with regards to both uterine fluid and blood serum concentrations of the other cytokines. CONCLUSION: Post-mating administration of anti-IL-10 and anti-TGFβ antibodies in mice may not have any effect on conception rates.
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spelling pubmed-44100392015-04-27 The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice Risvanli, Ali Godekmerdan, Ahmet Int J Fertil Steril Original Article BACKGROUND: In fertility studies, it has been shown that transforming growth factor β (TGFβ) and interlukin 10 (IL-10) play very important roles in implantation, maternal immune tolerance, placentation and fetal development, and the release beginning of release for fetal and postnatal death. The present study aims to determine the effects of the postmating administration of neutralizing antibodies against IL-10 and TGFβ, which significantly impact pregnancy in females and the conception rates in mice via assessments of blood serum and uterine fluid concentrations of IL-2, IL-4, IL-6, IL-10, IL-17, interferon γ (IFNγ), Tumor necrosis factor α (TNFα), and TGFβ. MATERIALS AND METHODS: In this experimental study, 21 BALB/c strain female mice were mated and randomly divided into three groups. The mice in the first group were selected as the control group. The second group of animals was injected with 0.5 mg of anti-IL-10 after mating, while those in the third group were intraperitoneally injected with 0.5 mg of anti-TGFβ. The animals in all groups were decapitated on the 13thday after mating and their blood samples were taken. The uteri were removed to determine pregnancy. The mice’s uterine irrigation fluids were also obtained. We used the multiplex immunoassay technique to determine the cytokine concentrations in uterine fluid and blood serum of the mice. RESULTS: We observed no intergroup difference with respect to conception rates. A comparison of the cytokine concentrations in the uterine fluids of pregnant mice revealed higher TGFβ concentrations (p<0.01) in the second group injected with the anti-IL-10 antibody compared with the other groups. There was no difference detected in pregnant animals with regards to both uterine fluid and blood serum concentrations of the other cytokines. CONCLUSION: Post-mating administration of anti-IL-10 and anti-TGFβ antibodies in mice may not have any effect on conception rates. Royan Institute 2015 2015-04-21 /pmc/articles/PMC4410039/ /pubmed/25918594 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Risvanli, Ali
Godekmerdan, Ahmet
The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title_full The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title_fullStr The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title_full_unstemmed The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title_short The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice
title_sort effects of post-mating administration of anti-il-10 and anti-tgfß on conception rates in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410039/
https://www.ncbi.nlm.nih.gov/pubmed/25918594
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