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Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa

To reach and fertilize the egg, mammalian spermatozoa change their flagellar movement in the female reproductive tract, named hyperactivation. The biochemical analyses of the hyperactivated movement using demembranated spermatozoa defined the factors inducing this peculiar movement; namely, large as...

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Autor principal: ISHIJIMA, Sumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japan Academy 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410089/
https://www.ncbi.nlm.nih.gov/pubmed/25765012
http://dx.doi.org/10.2183/pjab.91.99
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author ISHIJIMA, Sumio
author_facet ISHIJIMA, Sumio
author_sort ISHIJIMA, Sumio
collection PubMed
description To reach and fertilize the egg, mammalian spermatozoa change their flagellar movement in the female reproductive tract, named hyperactivation. The biochemical analyses of the hyperactivated movement using demembranated spermatozoa defined the factors inducing this peculiar movement; namely, large asymmetrical flagellar movement observed in the early stage of the hyperactivation was induced with a high Ca(2+) concentration while large symmetrical flagellar movement in the late stage of the hyperactivation was generated with low Ca(2+) and high cAMP concentrations. Under these conditions, the microtubule sliding of bull sperm flagella was investigated by disintegrating the sperm flagella with MgATP(2−) after extracting their plasma membrane and mitochondria. The large asymmetrical flagellar movement was caused by a long sliding displacement of a fiber of the doublet microtubules. On the other hand, the large symmetrical flagellar movement was generated by a large amount of microtubule sliding by many doublet microtubules.
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spelling pubmed-44100892015-05-27 Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa ISHIJIMA, Sumio Proc Jpn Acad Ser B Phys Biol Sci Original Article To reach and fertilize the egg, mammalian spermatozoa change their flagellar movement in the female reproductive tract, named hyperactivation. The biochemical analyses of the hyperactivated movement using demembranated spermatozoa defined the factors inducing this peculiar movement; namely, large asymmetrical flagellar movement observed in the early stage of the hyperactivation was induced with a high Ca(2+) concentration while large symmetrical flagellar movement in the late stage of the hyperactivation was generated with low Ca(2+) and high cAMP concentrations. Under these conditions, the microtubule sliding of bull sperm flagella was investigated by disintegrating the sperm flagella with MgATP(2−) after extracting their plasma membrane and mitochondria. The large asymmetrical flagellar movement was caused by a long sliding displacement of a fiber of the doublet microtubules. On the other hand, the large symmetrical flagellar movement was generated by a large amount of microtubule sliding by many doublet microtubules. The Japan Academy 2015-03-11 /pmc/articles/PMC4410089/ /pubmed/25765012 http://dx.doi.org/10.2183/pjab.91.99 Text en © 2015 The Japan Academy This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
ISHIJIMA, Sumio
Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title_full Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title_fullStr Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title_full_unstemmed Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title_short Ca(2+) and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
title_sort ca(2+) and camp regulations of microtubule sliding in hyperactivated motility of bull spermatozoa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410089/
https://www.ncbi.nlm.nih.gov/pubmed/25765012
http://dx.doi.org/10.2183/pjab.91.99
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