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Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes
We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410207/ https://www.ncbi.nlm.nih.gov/pubmed/25837344 http://dx.doi.org/10.3390/ijerph120403633 |
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author | Li, Qing Kobayashi, Maiko Kawada, Tomoyuki |
author_facet | Li, Qing Kobayashi, Maiko Kawada, Tomoyuki |
author_sort | Li, Qing |
collection | PubMed |
description | We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c. |
format | Online Article Text |
id | pubmed-4410207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44102072015-05-05 Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes Li, Qing Kobayashi, Maiko Kawada, Tomoyuki Int J Environ Res Public Health Article We previously found that carbamate pesticides induced significant apoptosis in human natural killer cells. To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. Apoptosis was determined by FITC-Annexin-V/PI staining. To explore the mechanism of apoptosis, intracellular levels of active caspase 3 and mitochondrial cytochrome-c release were determined by flow cytometry. We found that thiram, ziram, maneb and carbaryl also induced apoptosis in a time- and dose-dependent manner in the human T cells. However, the strength of the apoptosis-inducing effect differed among the pesticides, with the: thiram > ziram > maneb > carbaryl. Moreover, thiram significantly increased the intracellular level of active caspase 3 and caspase inhibitors significantly inhibited apoptosis. Thiram also significantly caused mitochondrial cytochrome-c release. These findings indicate that carbamate pesticides can induce apoptosis in human T cells, and the apoptosis is mediated by the activation of caspases and the release of mitochondrial cytochrome-c. MDPI 2015-04-01 2015-04 /pmc/articles/PMC4410207/ /pubmed/25837344 http://dx.doi.org/10.3390/ijerph120403633 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Qing Kobayashi, Maiko Kawada, Tomoyuki Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title | Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title_full | Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title_fullStr | Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title_full_unstemmed | Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title_short | Carbamate Pesticide-Induced Apoptosis in Human T Lymphocytes |
title_sort | carbamate pesticide-induced apoptosis in human t lymphocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410207/ https://www.ncbi.nlm.nih.gov/pubmed/25837344 http://dx.doi.org/10.3390/ijerph120403633 |
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