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Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation

Mucin 1 (Muc1) is an integral transmembrane mucin glycoprotein expressed on the apical surface of most epithelia. It is considered to be a barrier to the regulation of embryo implantation by inhibiting attachment of the embryo to the endometrium. Therefore, loss of Muc1 on the surface of uterine epi...

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Autores principales: INYAWILERT, Wilasinee, FU, Tzu-Yen, LIN, Chun-Ting, TANG, Pin-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410312/
https://www.ncbi.nlm.nih.gov/pubmed/25739861
http://dx.doi.org/10.1262/jrd.2014-106
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author INYAWILERT, Wilasinee
FU, Tzu-Yen
LIN, Chun-Ting
TANG, Pin-Chi
author_facet INYAWILERT, Wilasinee
FU, Tzu-Yen
LIN, Chun-Ting
TANG, Pin-Chi
author_sort INYAWILERT, Wilasinee
collection PubMed
description Mucin 1 (Muc1) is an integral transmembrane mucin glycoprotein expressed on the apical surface of most epithelia. It is considered to be a barrier to the regulation of embryo implantation by inhibiting attachment of the embryo to the endometrium. Therefore, loss of Muc1 on the surface of uterine epithelial cells is necessary for embryo implantation. Studies have demonstrated that microRNAs (miRNAs) play a key role in enhancing embryo implantation in mammals. In this study, we investigated the regulatory role of two miRNAs (let-7a and let-7b) on the expression of Muc1 in mouse uteri during implantation. Western blotting indicated that Muc1 expression was highest on day1 of pregnancy and constantly decreased thereafter until day 4. In contrast to Muc1 expression, increased expression of let-7a and let-7b was evident on day 4 of pregnancy as measured by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). We demonstrated direct binding of let-7a and let-7b to the 3’untranslated region of muc1. Furthermore, Muc1 expression was suppressed after transfection of mouse uterine epithelial cells isolated from day 1 of pregnancy with let-7a and let-7b. In summary, the present study provides evidence that Muc1 is a direct target of let-7a and let-7b. Additionally, the current study suggests that miRNAs are novel targets which can be used to facilitate a successful pregnancy and repair implantation failure.
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spelling pubmed-44103122015-04-28 Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation INYAWILERT, Wilasinee FU, Tzu-Yen LIN, Chun-Ting TANG, Pin-Chi J Reprod Dev Original Article Mucin 1 (Muc1) is an integral transmembrane mucin glycoprotein expressed on the apical surface of most epithelia. It is considered to be a barrier to the regulation of embryo implantation by inhibiting attachment of the embryo to the endometrium. Therefore, loss of Muc1 on the surface of uterine epithelial cells is necessary for embryo implantation. Studies have demonstrated that microRNAs (miRNAs) play a key role in enhancing embryo implantation in mammals. In this study, we investigated the regulatory role of two miRNAs (let-7a and let-7b) on the expression of Muc1 in mouse uteri during implantation. Western blotting indicated that Muc1 expression was highest on day1 of pregnancy and constantly decreased thereafter until day 4. In contrast to Muc1 expression, increased expression of let-7a and let-7b was evident on day 4 of pregnancy as measured by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). We demonstrated direct binding of let-7a and let-7b to the 3’untranslated region of muc1. Furthermore, Muc1 expression was suppressed after transfection of mouse uterine epithelial cells isolated from day 1 of pregnancy with let-7a and let-7b. In summary, the present study provides evidence that Muc1 is a direct target of let-7a and let-7b. Additionally, the current study suggests that miRNAs are novel targets which can be used to facilitate a successful pregnancy and repair implantation failure. The Society for Reproduction and Development 2015-01-26 2015-04 /pmc/articles/PMC4410312/ /pubmed/25739861 http://dx.doi.org/10.1262/jrd.2014-106 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
INYAWILERT, Wilasinee
FU, Tzu-Yen
LIN, Chun-Ting
TANG, Pin-Chi
Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title_full Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title_fullStr Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title_full_unstemmed Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title_short Let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
title_sort let-7-mediated suppression of mucin 1 expression in the mouse uterus during embryo implantation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410312/
https://www.ncbi.nlm.nih.gov/pubmed/25739861
http://dx.doi.org/10.1262/jrd.2014-106
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