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Recombinant α-NAcetylgalactosaminidase from Marine Bacterium-Modifying A Erythrocyte Antigens

A plasmid based on pET-40b was constructed to synthesize recombinant α-N-acetylgalactosaminidase of the marine bacterium Arenibacter latericius KMM 426T (α-AlNaGal) in Escherichia coli cells. The yield of α-Al- NaGal attains 10 mg/ml with activity of 49.7 ± 1.3 U at 16°C, concentration of inductor 2...

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Detalles Bibliográficos
Autores principales:	Balabanova, L. A., Golotin, V. A., Bakunina, I. Y., Slepchenko, L. V., Isakov, V. V., Podvolotskaya, A. B., Rasskazov, V. A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	A.I. Gordeyev 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410403/ https://www.ncbi.nlm.nih.gov/pubmed/25927009

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