Cargando…

Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model

BACKGROUND: Caesalpinia sappan L. extracts exhibit great therapeutic potential, and have been shown to have analgesic and anti-inflammatory properties. This study aimed to understand the anti-rheumatoid activity of brazilin that was isolated from ethyl acetate extract of C. sappan L. The evaluations...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Eui-Gil, Han, Kook-Il, Hwang, Seon Gu, Kwon, Hyun-Jung, Patnaik, Bharat Bhusan, Kim, Yong Hyun, Han, Man-Deuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410491/
https://www.ncbi.nlm.nih.gov/pubmed/25896410
http://dx.doi.org/10.1186/s12906-015-0648-x
_version_ 1782368335560704000
author Jung, Eui-Gil
Han, Kook-Il
Hwang, Seon Gu
Kwon, Hyun-Jung
Patnaik, Bharat Bhusan
Kim, Yong Hyun
Han, Man-Deuk
author_facet Jung, Eui-Gil
Han, Kook-Il
Hwang, Seon Gu
Kwon, Hyun-Jung
Patnaik, Bharat Bhusan
Kim, Yong Hyun
Han, Man-Deuk
author_sort Jung, Eui-Gil
collection PubMed
description BACKGROUND: Caesalpinia sappan L. extracts exhibit great therapeutic potential, and have been shown to have analgesic and anti-inflammatory properties. This study aimed to understand the anti-rheumatoid activity of brazilin that was isolated from ethyl acetate extract of C. sappan L. The evaluations were conducted in mice with type-II collagen-induced arthritis (CIA). METHODS: Brazilin was purified via preparative HPLC and identified by mass spectrometry and (1)H/(13)C NMR analysis. DBA/1J mice were divided into four groups (n = 10). Three groups of mice received intradermal injections of inducer bovine type-II collagen (BTIIC; 2 mg/ml in 0.05 ml acetic acid) and 0.1 ml of booster complete Freund’s adjuvant (CFA). A second injection of BTIIC with booster incomplete Freund’s adjuvant (ICFA) was given subsequently after 21 days. On 22nd day, purified brazilin (10 mg/kg body weight) or the disease-modifying anti-rheumatic drug methotrexate (3 mg/kg body weight) was administered intraperitoneally daily or every three days for 21 days, respectively to two groups of mice. At the 42nd day, mice sera were collected, and the levels of pro-inflammatory cytokines and stress enzyme markers in serum were measured using standard immunoassay methods. The microstructure and morphometric analyses of the bones were assessed using high-resolution microfocal computed tomography. RESULTS: Brazilin isolated from C. sappan reduced the arthritis index score and the extent of acute inflammatory paw edema in CIA-mice. The bone mineral density was significantly (p < 0.05) lower in only-CIA mice, and appeared to increase commensurate with methotrexate and brazilin administration. Brazilin prevented joint destruction, surface erosion, and enhanced bone formation as revealed by microstructural examinations. Brazilin markedly attenuated mouse CIA and reduced the serum levels of inflammatory cytokines including TNF-α, IL-1β, and IL-6. CONCLUSIONS: Brazilin purified from C. sappan L. shows protective efficacy in CIA mouse, and may be useful to treat chronic inflammatory disorders including rheumatoid arthritis.
format Online
Article
Text
id pubmed-4410491
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-44104912015-04-28 Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model Jung, Eui-Gil Han, Kook-Il Hwang, Seon Gu Kwon, Hyun-Jung Patnaik, Bharat Bhusan Kim, Yong Hyun Han, Man-Deuk BMC Complement Altern Med Research Article BACKGROUND: Caesalpinia sappan L. extracts exhibit great therapeutic potential, and have been shown to have analgesic and anti-inflammatory properties. This study aimed to understand the anti-rheumatoid activity of brazilin that was isolated from ethyl acetate extract of C. sappan L. The evaluations were conducted in mice with type-II collagen-induced arthritis (CIA). METHODS: Brazilin was purified via preparative HPLC and identified by mass spectrometry and (1)H/(13)C NMR analysis. DBA/1J mice were divided into four groups (n = 10). Three groups of mice received intradermal injections of inducer bovine type-II collagen (BTIIC; 2 mg/ml in 0.05 ml acetic acid) and 0.1 ml of booster complete Freund’s adjuvant (CFA). A second injection of BTIIC with booster incomplete Freund’s adjuvant (ICFA) was given subsequently after 21 days. On 22nd day, purified brazilin (10 mg/kg body weight) or the disease-modifying anti-rheumatic drug methotrexate (3 mg/kg body weight) was administered intraperitoneally daily or every three days for 21 days, respectively to two groups of mice. At the 42nd day, mice sera were collected, and the levels of pro-inflammatory cytokines and stress enzyme markers in serum were measured using standard immunoassay methods. The microstructure and morphometric analyses of the bones were assessed using high-resolution microfocal computed tomography. RESULTS: Brazilin isolated from C. sappan reduced the arthritis index score and the extent of acute inflammatory paw edema in CIA-mice. The bone mineral density was significantly (p < 0.05) lower in only-CIA mice, and appeared to increase commensurate with methotrexate and brazilin administration. Brazilin prevented joint destruction, surface erosion, and enhanced bone formation as revealed by microstructural examinations. Brazilin markedly attenuated mouse CIA and reduced the serum levels of inflammatory cytokines including TNF-α, IL-1β, and IL-6. CONCLUSIONS: Brazilin purified from C. sappan L. shows protective efficacy in CIA mouse, and may be useful to treat chronic inflammatory disorders including rheumatoid arthritis. BioMed Central 2015-04-22 /pmc/articles/PMC4410491/ /pubmed/25896410 http://dx.doi.org/10.1186/s12906-015-0648-x Text en © Jung et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jung, Eui-Gil
Han, Kook-Il
Hwang, Seon Gu
Kwon, Hyun-Jung
Patnaik, Bharat Bhusan
Kim, Yong Hyun
Han, Man-Deuk
Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title_full Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title_fullStr Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title_full_unstemmed Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title_short Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model
title_sort brazilin isolated from caesalpinia sappan l. inhibits rheumatoid arthritis activity in a type-ii collagen induced arthritis mouse model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410491/
https://www.ncbi.nlm.nih.gov/pubmed/25896410
http://dx.doi.org/10.1186/s12906-015-0648-x
work_keys_str_mv AT jungeuigil brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT hankookil brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT hwangseongu brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT kwonhyunjung brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT patnaikbharatbhusan brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT kimyonghyun brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel
AT hanmandeuk brazilinisolatedfromcaesalpiniasappanlinhibitsrheumatoidarthritisactivityinatypeiicollageninducedarthritismousemodel