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The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells
BACKGROUND: Breast cancer-related mortality increases annually. The efficacy of current breast cancer treatments is limited, and they have numerous side effects and permit high recurrence. Patients with estrogen receptor (ER)-negative or triple-negative breast cancer are particularly difficult to tr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410586/ https://www.ncbi.nlm.nih.gov/pubmed/25885960 http://dx.doi.org/10.1186/s12906-015-0592-9 |
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author | Hsu, Ren-Jun Hsu, Yao-Chin Chen, Shu-Pin Fu, Chia-Lynn Yu, Jyh-Cherng Chang, Fung-Wei Chen, Ying-Hsin Liu, Jui-Ming Ho, Jar-Yi Yu, Cheng-Ping |
author_facet | Hsu, Ren-Jun Hsu, Yao-Chin Chen, Shu-Pin Fu, Chia-Lynn Yu, Jyh-Cherng Chang, Fung-Wei Chen, Ying-Hsin Liu, Jui-Ming Ho, Jar-Yi Yu, Cheng-Ping |
author_sort | Hsu, Ren-Jun |
collection | PubMed |
description | BACKGROUND: Breast cancer-related mortality increases annually. The efficacy of current breast cancer treatments is limited, and they have numerous side effects and permit high recurrence. Patients with estrogen receptor (ER)-negative or triple-negative breast cancer are particularly difficult to treat. Treatment for this type of cancer is lacking, and its prognosis is poor, necessitating the search for alternative treatments. METHODS: This study screened Chinese herb Hibiscus syriacus extracts and identified a novel anti-cancer drug for patients with ER-negative breast cancer. The inhibitory effects on cell viability and migration were evaluated for each compound, and the molecular regulatory effects were evaluated on both mRNA and protein levels. RESULT: We found several triterpenoids including betulin (K02) and its derivatives (K03, K04, and K06) from H. syriacus inhibited human triple-negative breast cancer cell viability and migration but revealed smaller cytotoxic effects on normal mammalian epithelial cells. Betulin and its derivatives induced apoptosis by activating apoptosis-related genes. In addition, they activated p21 expression, which induced cell cycle arrest in breast cancer cells. Betulin (K02) and betulinic acid (K06) had stronger inhibitory effects on cell viability and migration than K03 and K04. CONCLUSIONS: H. syriacus extracts might inhibit breast cancer cell viability and induce apoptosis by activating p53 family regulated pathways and inhibiting AKT activation. H. syriacus extracts may provide important insight into the development of novel alternative therapies for breast cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0592-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4410586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44105862015-04-28 The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells Hsu, Ren-Jun Hsu, Yao-Chin Chen, Shu-Pin Fu, Chia-Lynn Yu, Jyh-Cherng Chang, Fung-Wei Chen, Ying-Hsin Liu, Jui-Ming Ho, Jar-Yi Yu, Cheng-Ping BMC Complement Altern Med Research Article BACKGROUND: Breast cancer-related mortality increases annually. The efficacy of current breast cancer treatments is limited, and they have numerous side effects and permit high recurrence. Patients with estrogen receptor (ER)-negative or triple-negative breast cancer are particularly difficult to treat. Treatment for this type of cancer is lacking, and its prognosis is poor, necessitating the search for alternative treatments. METHODS: This study screened Chinese herb Hibiscus syriacus extracts and identified a novel anti-cancer drug for patients with ER-negative breast cancer. The inhibitory effects on cell viability and migration were evaluated for each compound, and the molecular regulatory effects were evaluated on both mRNA and protein levels. RESULT: We found several triterpenoids including betulin (K02) and its derivatives (K03, K04, and K06) from H. syriacus inhibited human triple-negative breast cancer cell viability and migration but revealed smaller cytotoxic effects on normal mammalian epithelial cells. Betulin and its derivatives induced apoptosis by activating apoptosis-related genes. In addition, they activated p21 expression, which induced cell cycle arrest in breast cancer cells. Betulin (K02) and betulinic acid (K06) had stronger inhibitory effects on cell viability and migration than K03 and K04. CONCLUSIONS: H. syriacus extracts might inhibit breast cancer cell viability and induce apoptosis by activating p53 family regulated pathways and inhibiting AKT activation. H. syriacus extracts may provide important insight into the development of novel alternative therapies for breast cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0592-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-14 /pmc/articles/PMC4410586/ /pubmed/25885960 http://dx.doi.org/10.1186/s12906-015-0592-9 Text en © Hsu et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hsu, Ren-Jun Hsu, Yao-Chin Chen, Shu-Pin Fu, Chia-Lynn Yu, Jyh-Cherng Chang, Fung-Wei Chen, Ying-Hsin Liu, Jui-Ming Ho, Jar-Yi Yu, Cheng-Ping The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title | The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title_full | The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title_fullStr | The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title_full_unstemmed | The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title_short | The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
title_sort | triterpenoids of hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4410586/ https://www.ncbi.nlm.nih.gov/pubmed/25885960 http://dx.doi.org/10.1186/s12906-015-0592-9 |
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