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Interplay between Endometriosis and Pregnancy in a Mouse Model
OBJECTIVES: To evaluate the effect of endometriosis on fertility and the levels of the IL-2 and IFN-γ in the peritoneal fluid in a mouse model; to evaluate the effect of pregnancy on endometriotic lesion growth, apoptosis and cell proliferation. STUDY DESIGN: Two month old C57BL/6 female mice underw...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411153/ https://www.ncbi.nlm.nih.gov/pubmed/25915402 http://dx.doi.org/10.1371/journal.pone.0124900 |
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author | Bilotas, Mariela Andrea Olivares, Carla Noemí Ricci, Analía Gabriela Baston, Juan Ignacio Bengochea, Tatiana Soledad Meresman, Gabriela Fabiana Barañao, Rosa Inés |
author_facet | Bilotas, Mariela Andrea Olivares, Carla Noemí Ricci, Analía Gabriela Baston, Juan Ignacio Bengochea, Tatiana Soledad Meresman, Gabriela Fabiana Barañao, Rosa Inés |
author_sort | Bilotas, Mariela Andrea |
collection | PubMed |
description | OBJECTIVES: To evaluate the effect of endometriosis on fertility and the levels of the IL-2 and IFN-γ in the peritoneal fluid in a mouse model; to evaluate the effect of pregnancy on endometriotic lesion growth, apoptosis and cell proliferation. STUDY DESIGN: Two month old C57BL/6 female mice underwent either a surgical procedure to induce endometriosis or a sham surgery. Four weeks after surgery mice were mated and sacrificed at day 18 of pregnancy. Number of implantation sites, fetuses and fetal weight were recorded. Endometriotic lesions were counted, measured, excised and fixed. Apoptosis and cell proliferation were evaluated in lesions by TUNEL and immunohistochemistry for PCNA respectively. Levels of IL-2 and IFN-γ were assessed by ELISA in the peritoneal fluid. RESULTS: Pregnancy rate (i.e. pregnant mice/N) decreased in mice with endometriosis. However there were no significant differences in resorption rate, litter size and pup weight between groups. IFN-γ augmented in endometriosis mice independently of pregnancy outcome. Additionally IFN-γ increased in pregnant endometriosis mice compared to pregnant sham animals. While IFN-γ increased in non pregnant versus pregnant mice in the sham group, IL-2 was increased in non pregnant mice in the endometriosis group. The size of endometriotic lesions increased in pregnant mice while apoptosis increased in the stroma and cell proliferation decreased in the epithelium of these lesions. Additionally, leukocyte infiltration, necrosis and decidualization were increased in the same lesions. CONCLUSIONS: Pregnancy rate is reduced in this mouse model of endometriosis. Levels of IL-2 are increased in the peritoneal fluid of mice with endometriosis suggesting a role of this cytokine in infertility related to this disease. The size of endometriotic lesions is increased in pregnant mice; however pregnancy has a beneficial effect on lesions by decreasing cell proliferation and by increasing apoptosis, decidualization and necrosis. |
format | Online Article Text |
id | pubmed-4411153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44111532015-05-07 Interplay between Endometriosis and Pregnancy in a Mouse Model Bilotas, Mariela Andrea Olivares, Carla Noemí Ricci, Analía Gabriela Baston, Juan Ignacio Bengochea, Tatiana Soledad Meresman, Gabriela Fabiana Barañao, Rosa Inés PLoS One Research Article OBJECTIVES: To evaluate the effect of endometriosis on fertility and the levels of the IL-2 and IFN-γ in the peritoneal fluid in a mouse model; to evaluate the effect of pregnancy on endometriotic lesion growth, apoptosis and cell proliferation. STUDY DESIGN: Two month old C57BL/6 female mice underwent either a surgical procedure to induce endometriosis or a sham surgery. Four weeks after surgery mice were mated and sacrificed at day 18 of pregnancy. Number of implantation sites, fetuses and fetal weight were recorded. Endometriotic lesions were counted, measured, excised and fixed. Apoptosis and cell proliferation were evaluated in lesions by TUNEL and immunohistochemistry for PCNA respectively. Levels of IL-2 and IFN-γ were assessed by ELISA in the peritoneal fluid. RESULTS: Pregnancy rate (i.e. pregnant mice/N) decreased in mice with endometriosis. However there were no significant differences in resorption rate, litter size and pup weight between groups. IFN-γ augmented in endometriosis mice independently of pregnancy outcome. Additionally IFN-γ increased in pregnant endometriosis mice compared to pregnant sham animals. While IFN-γ increased in non pregnant versus pregnant mice in the sham group, IL-2 was increased in non pregnant mice in the endometriosis group. The size of endometriotic lesions increased in pregnant mice while apoptosis increased in the stroma and cell proliferation decreased in the epithelium of these lesions. Additionally, leukocyte infiltration, necrosis and decidualization were increased in the same lesions. CONCLUSIONS: Pregnancy rate is reduced in this mouse model of endometriosis. Levels of IL-2 are increased in the peritoneal fluid of mice with endometriosis suggesting a role of this cytokine in infertility related to this disease. The size of endometriotic lesions is increased in pregnant mice; however pregnancy has a beneficial effect on lesions by decreasing cell proliferation and by increasing apoptosis, decidualization and necrosis. Public Library of Science 2015-04-27 /pmc/articles/PMC4411153/ /pubmed/25915402 http://dx.doi.org/10.1371/journal.pone.0124900 Text en © 2015 Bilotas et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bilotas, Mariela Andrea Olivares, Carla Noemí Ricci, Analía Gabriela Baston, Juan Ignacio Bengochea, Tatiana Soledad Meresman, Gabriela Fabiana Barañao, Rosa Inés Interplay between Endometriosis and Pregnancy in a Mouse Model |
title | Interplay between Endometriosis and Pregnancy in a Mouse Model |
title_full | Interplay between Endometriosis and Pregnancy in a Mouse Model |
title_fullStr | Interplay between Endometriosis and Pregnancy in a Mouse Model |
title_full_unstemmed | Interplay between Endometriosis and Pregnancy in a Mouse Model |
title_short | Interplay between Endometriosis and Pregnancy in a Mouse Model |
title_sort | interplay between endometriosis and pregnancy in a mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411153/ https://www.ncbi.nlm.nih.gov/pubmed/25915402 http://dx.doi.org/10.1371/journal.pone.0124900 |
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