Cargando…
In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411679/ https://www.ncbi.nlm.nih.gov/pubmed/25856635 http://dx.doi.org/10.3390/v7041832 |
_version_ | 1782368518570770432 |
---|---|
author | Thapa, Simrika Abdul Cader, Mohamed Sarjoon Murugananthan, Kalamathy Nagy, Eva Sharif, Shayan Czub, Markus Abdul-Careem, Mohamed Faizal |
author_facet | Thapa, Simrika Abdul Cader, Mohamed Sarjoon Murugananthan, Kalamathy Nagy, Eva Sharif, Shayan Czub, Markus Abdul-Careem, Mohamed Faizal |
author_sort | Thapa, Simrika |
collection | PubMed |
description | Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens. |
format | Online Article Text |
id | pubmed-4411679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44116792015-05-06 In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity Thapa, Simrika Abdul Cader, Mohamed Sarjoon Murugananthan, Kalamathy Nagy, Eva Sharif, Shayan Czub, Markus Abdul-Careem, Mohamed Faizal Viruses Article Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens. MDPI 2015-04-08 /pmc/articles/PMC4411679/ /pubmed/25856635 http://dx.doi.org/10.3390/v7041832 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thapa, Simrika Abdul Cader, Mohamed Sarjoon Murugananthan, Kalamathy Nagy, Eva Sharif, Shayan Czub, Markus Abdul-Careem, Mohamed Faizal In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title | In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title_full | In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title_fullStr | In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title_full_unstemmed | In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title_short | In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity |
title_sort | in ovo delivery of cpg dna reduces avian infectious laryngotracheitis virus induced mortality and morbidity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411679/ https://www.ncbi.nlm.nih.gov/pubmed/25856635 http://dx.doi.org/10.3390/v7041832 |
work_keys_str_mv | AT thapasimrika inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT abdulcadermohamedsarjoon inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT murugananthankalamathy inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT nagyeva inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT sharifshayan inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT czubmarkus inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity AT abdulcareemmohamedfaizal inovodeliveryofcpgdnareducesavianinfectiouslaryngotracheitisvirusinducedmortalityandmorbidity |