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In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity

Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo...

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Autores principales: Thapa, Simrika, Abdul Cader, Mohamed Sarjoon, Murugananthan, Kalamathy, Nagy, Eva, Sharif, Shayan, Czub, Markus, Abdul-Careem, Mohamed Faizal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411679/
https://www.ncbi.nlm.nih.gov/pubmed/25856635
http://dx.doi.org/10.3390/v7041832
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author Thapa, Simrika
Abdul Cader, Mohamed Sarjoon
Murugananthan, Kalamathy
Nagy, Eva
Sharif, Shayan
Czub, Markus
Abdul-Careem, Mohamed Faizal
author_facet Thapa, Simrika
Abdul Cader, Mohamed Sarjoon
Murugananthan, Kalamathy
Nagy, Eva
Sharif, Shayan
Czub, Markus
Abdul-Careem, Mohamed Faizal
author_sort Thapa, Simrika
collection PubMed
description Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens.
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spelling pubmed-44116792015-05-06 In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity Thapa, Simrika Abdul Cader, Mohamed Sarjoon Murugananthan, Kalamathy Nagy, Eva Sharif, Shayan Czub, Markus Abdul-Careem, Mohamed Faizal Viruses Article Endosomal toll-like receptor-21 and -9 sense CpG DNA activating production of pro-inflammatory mediators with antimicrobial effects. Here, we investigated the induction of antiviral response of in ovo delivered CpG DNA against infectious laryngotracheitis virus (ILTV) infection. We found that in ovo delivered CpG DNA significantly reduces ILTV infection pre-hatch correlating with the expression of IL-1β and increase of macrophages in lungs. As assessed in vitro, CpG DNA stimulated avian macrophages could be a potential source of IL-1β and other pro-inflammatory mediators. Since we also found that in ovo CpG DNA delivery maintains increased macrophages in the lungs post-hatch, we infected the chickens on the day of hatch with ILTV. We found that in ovo delivered CpG DNA significantly reduces mortality and morbidity resulting from ILTV infection encountered post-hatch. Thus, CpG DNA can be a candidate innate immune stimulant worthy of further investigation for the control of ILTV infection in chickens. MDPI 2015-04-08 /pmc/articles/PMC4411679/ /pubmed/25856635 http://dx.doi.org/10.3390/v7041832 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thapa, Simrika
Abdul Cader, Mohamed Sarjoon
Murugananthan, Kalamathy
Nagy, Eva
Sharif, Shayan
Czub, Markus
Abdul-Careem, Mohamed Faizal
In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title_full In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title_fullStr In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title_full_unstemmed In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title_short In Ovo Delivery of CpG DNA Reduces Avian Infectious Laryngotracheitis Virus Induced Mortality and Morbidity
title_sort in ovo delivery of cpg dna reduces avian infectious laryngotracheitis virus induced mortality and morbidity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411679/
https://www.ncbi.nlm.nih.gov/pubmed/25856635
http://dx.doi.org/10.3390/v7041832
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