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Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411693/ https://www.ncbi.nlm.nih.gov/pubmed/25912716 http://dx.doi.org/10.3390/v7042126 |
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author | Hatcher, Eneida L. Wang, Chunlin Lefkowitz, Elliot J. |
author_facet | Hatcher, Eneida L. Wang, Chunlin Lefkowitz, Elliot J. |
author_sort | Hatcher, Eneida L. |
collection | PubMed |
description | To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the early stop mutations (ESMs) leading to gene truncation or fragmentation were analyzed. Of all the ESMs present in these poxvirus genomes, over 65% co-localized with microsatellites—simple sequence nucleotide repeats. On average, microsatellites comprise 24% of the nucleotide sequence of these poxvirus genomes. These simple repeats have been shown to exhibit high rates of variation, and represent a target for poxvirus protein variation, gene truncation, and reductive evolution. |
format | Online Article Text |
id | pubmed-4411693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44116932015-05-06 Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites Hatcher, Eneida L. Wang, Chunlin Lefkowitz, Elliot J. Viruses Article To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the early stop mutations (ESMs) leading to gene truncation or fragmentation were analyzed. Of all the ESMs present in these poxvirus genomes, over 65% co-localized with microsatellites—simple sequence nucleotide repeats. On average, microsatellites comprise 24% of the nucleotide sequence of these poxvirus genomes. These simple repeats have been shown to exhibit high rates of variation, and represent a target for poxvirus protein variation, gene truncation, and reductive evolution. MDPI 2015-04-22 /pmc/articles/PMC4411693/ /pubmed/25912716 http://dx.doi.org/10.3390/v7042126 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hatcher, Eneida L. Wang, Chunlin Lefkowitz, Elliot J. Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title | Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title_full | Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title_fullStr | Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title_full_unstemmed | Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title_short | Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites |
title_sort | genome variability and gene content in chordopoxviruses: dependence on microsatellites |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411693/ https://www.ncbi.nlm.nih.gov/pubmed/25912716 http://dx.doi.org/10.3390/v7042126 |
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