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Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites

To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the...

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Autores principales: Hatcher, Eneida L., Wang, Chunlin, Lefkowitz, Elliot J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411693/
https://www.ncbi.nlm.nih.gov/pubmed/25912716
http://dx.doi.org/10.3390/v7042126
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author Hatcher, Eneida L.
Wang, Chunlin
Lefkowitz, Elliot J.
author_facet Hatcher, Eneida L.
Wang, Chunlin
Lefkowitz, Elliot J.
author_sort Hatcher, Eneida L.
collection PubMed
description To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the early stop mutations (ESMs) leading to gene truncation or fragmentation were analyzed. Of all the ESMs present in these poxvirus genomes, over 65% co-localized with microsatellites—simple sequence nucleotide repeats. On average, microsatellites comprise 24% of the nucleotide sequence of these poxvirus genomes. These simple repeats have been shown to exhibit high rates of variation, and represent a target for poxvirus protein variation, gene truncation, and reductive evolution.
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spelling pubmed-44116932015-05-06 Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites Hatcher, Eneida L. Wang, Chunlin Lefkowitz, Elliot J. Viruses Article To investigate gene loss in poxviruses belonging to the Chordopoxvirinae subfamily, we assessed the gene content of representative members of the subfamily, and determined whether individual genes present in each genome were intact, truncated, or fragmented. When nonintact genes were identified, the early stop mutations (ESMs) leading to gene truncation or fragmentation were analyzed. Of all the ESMs present in these poxvirus genomes, over 65% co-localized with microsatellites—simple sequence nucleotide repeats. On average, microsatellites comprise 24% of the nucleotide sequence of these poxvirus genomes. These simple repeats have been shown to exhibit high rates of variation, and represent a target for poxvirus protein variation, gene truncation, and reductive evolution. MDPI 2015-04-22 /pmc/articles/PMC4411693/ /pubmed/25912716 http://dx.doi.org/10.3390/v7042126 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hatcher, Eneida L.
Wang, Chunlin
Lefkowitz, Elliot J.
Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title_full Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title_fullStr Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title_full_unstemmed Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title_short Genome Variability and Gene Content in Chordopoxviruses: Dependence on Microsatellites
title_sort genome variability and gene content in chordopoxviruses: dependence on microsatellites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411693/
https://www.ncbi.nlm.nih.gov/pubmed/25912716
http://dx.doi.org/10.3390/v7042126
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