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Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen)
The process of amygdalin extraction from plum seeds was optimized using central composite design (CCD) and multilayer perceptron (MLP). The effect of time, ethanol concentration, solid-to-liquid ratio, and temperature on the amygdalin content in the extracts was estimated using both mathematical mod...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411975/ https://www.ncbi.nlm.nih.gov/pubmed/25972881 http://dx.doi.org/10.3389/fpls.2015.00276 |
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author | Savic, Ivan M. Nikolic, Vesna D. Savic-Gajic, Ivana M. Nikolic, Ljubisa B. Ibric, Svetlana R. Gajic, Dragoljub G. |
author_facet | Savic, Ivan M. Nikolic, Vesna D. Savic-Gajic, Ivana M. Nikolic, Ljubisa B. Ibric, Svetlana R. Gajic, Dragoljub G. |
author_sort | Savic, Ivan M. |
collection | PubMed |
description | The process of amygdalin extraction from plum seeds was optimized using central composite design (CCD) and multilayer perceptron (MLP). The effect of time, ethanol concentration, solid-to-liquid ratio, and temperature on the amygdalin content in the extracts was estimated using both mathematical models. The MLP 4-3-1 with exponential function in hidden layer and linear function in output layer was used for describing the extraction process. MLP model was more superior compared with CCD model due to better prediction ability. According to MLP model, the suggested optimal conditions are: time of 120 min, 100% (v/v) ethanol, solid-to liquid ratio of 1:25 (m/v) and temperature of 34.4°C. The predicted value of amygdalin content in the dried extract (25.42 g per 100 g) at these conditions was experimentally confirmed (25.30 g per 100 g of dried extract). Amygdalin (>90%) was isolated from the complex extraction mixture and structurally characterized by FT-IR, UV, and MS methods. |
format | Online Article Text |
id | pubmed-4411975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44119752015-05-13 Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) Savic, Ivan M. Nikolic, Vesna D. Savic-Gajic, Ivana M. Nikolic, Ljubisa B. Ibric, Svetlana R. Gajic, Dragoljub G. Front Plant Sci Plant Science The process of amygdalin extraction from plum seeds was optimized using central composite design (CCD) and multilayer perceptron (MLP). The effect of time, ethanol concentration, solid-to-liquid ratio, and temperature on the amygdalin content in the extracts was estimated using both mathematical models. The MLP 4-3-1 with exponential function in hidden layer and linear function in output layer was used for describing the extraction process. MLP model was more superior compared with CCD model due to better prediction ability. According to MLP model, the suggested optimal conditions are: time of 120 min, 100% (v/v) ethanol, solid-to liquid ratio of 1:25 (m/v) and temperature of 34.4°C. The predicted value of amygdalin content in the dried extract (25.42 g per 100 g) at these conditions was experimentally confirmed (25.30 g per 100 g of dried extract). Amygdalin (>90%) was isolated from the complex extraction mixture and structurally characterized by FT-IR, UV, and MS methods. Frontiers Media S.A. 2015-04-28 /pmc/articles/PMC4411975/ /pubmed/25972881 http://dx.doi.org/10.3389/fpls.2015.00276 Text en Copyright © 2015 Savic, Nikolic, Savic-Gajic, Nikolic, Ibric and Gajic. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Savic, Ivan M. Nikolic, Vesna D. Savic-Gajic, Ivana M. Nikolic, Ljubisa B. Ibric, Svetlana R. Gajic, Dragoljub G. Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title | Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title_full | Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title_fullStr | Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title_full_unstemmed | Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title_short | Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen) |
title_sort | optimization of technological procedure for amygdalin isolation from plum seeds (pruni domesticae semen) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411975/ https://www.ncbi.nlm.nih.gov/pubmed/25972881 http://dx.doi.org/10.3389/fpls.2015.00276 |
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