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Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene

The purpose of this study was to investigate the alternative splicing in equine cordon-bleu WH2 repeat protein-like 1 (COBLL1) gene that was identified in horse muscle and blood leukocytes, and to predict functional consequences of alternative splicing by bioinformatics analysis. In a previous study...

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Autores principales: Park, Jeong-Woong, Jang, Hyun-Jun, Shin, Sangsu, Cho, Hyun-Woo, Choi, Jae-Young, Kim, Nam-Young, Lee, Hak-Kyo, Do, Kyong-Tak, Song, Ki-Duk, Cho, Byung-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412984/
https://www.ncbi.nlm.nih.gov/pubmed/25925064
http://dx.doi.org/10.5713/ajas.14.0722
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author Park, Jeong-Woong
Jang, Hyun-Jun
Shin, Sangsu
Cho, Hyun-Woo
Choi, Jae-Young
Kim, Nam-Young
Lee, Hak-Kyo
Do, Kyong-Tak
Song, Ki-Duk
Cho, Byung-Wook
author_facet Park, Jeong-Woong
Jang, Hyun-Jun
Shin, Sangsu
Cho, Hyun-Woo
Choi, Jae-Young
Kim, Nam-Young
Lee, Hak-Kyo
Do, Kyong-Tak
Song, Ki-Duk
Cho, Byung-Wook
author_sort Park, Jeong-Woong
collection PubMed
description The purpose of this study was to investigate the alternative splicing in equine cordon-bleu WH2 repeat protein-like 1 (COBLL1) gene that was identified in horse muscle and blood leukocytes, and to predict functional consequences of alternative splicing by bioinformatics analysis. In a previous study, RNA-seq analysis predicted the presence of alternative spliced isoforms of equine COBLL1, namely COBLL1a as a long form and COBLL1b as a short form. In this study, we validated two isoforms of COBLL1 transcripts in horse tissues by the real-time polymerase chain reaction, and cloned them for Sanger sequencing. The sequencing results showed that the alternative splicing occurs at exon 9. Prediction of protein structure of these isoforms revealed three putative phosphorylation sites at the amino acid sequences encoded in exon 9, which is deleted in COBLL1b. In expression analysis, it was found that COBLL1b was expressed ubiquitously and equivalently in all the analyzed tissues, whereas COBLL1a showed strong expression in kidney, spinal cord and lung, moderate expression in heart and skeletal muscle, and low expression in thyroid and colon. In muscle, both COBLL1a and COBLL1b expression decreased after exercise. It is assumed that the regulation of COBLL1 expression may be important for regulating glucose level or switching of energy source, possibly through an insulin signaling pathway, in muscle after exercise. Further study is warranted to reveal the functional importance of COBLL1 on athletic performance in race horses.
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spelling pubmed-44129842015-06-01 Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene Park, Jeong-Woong Jang, Hyun-Jun Shin, Sangsu Cho, Hyun-Woo Choi, Jae-Young Kim, Nam-Young Lee, Hak-Kyo Do, Kyong-Tak Song, Ki-Duk Cho, Byung-Wook Asian-Australas J Anim Sci Article The purpose of this study was to investigate the alternative splicing in equine cordon-bleu WH2 repeat protein-like 1 (COBLL1) gene that was identified in horse muscle and blood leukocytes, and to predict functional consequences of alternative splicing by bioinformatics analysis. In a previous study, RNA-seq analysis predicted the presence of alternative spliced isoforms of equine COBLL1, namely COBLL1a as a long form and COBLL1b as a short form. In this study, we validated two isoforms of COBLL1 transcripts in horse tissues by the real-time polymerase chain reaction, and cloned them for Sanger sequencing. The sequencing results showed that the alternative splicing occurs at exon 9. Prediction of protein structure of these isoforms revealed three putative phosphorylation sites at the amino acid sequences encoded in exon 9, which is deleted in COBLL1b. In expression analysis, it was found that COBLL1b was expressed ubiquitously and equivalently in all the analyzed tissues, whereas COBLL1a showed strong expression in kidney, spinal cord and lung, moderate expression in heart and skeletal muscle, and low expression in thyroid and colon. In muscle, both COBLL1a and COBLL1b expression decreased after exercise. It is assumed that the regulation of COBLL1 expression may be important for regulating glucose level or switching of energy source, possibly through an insulin signaling pathway, in muscle after exercise. Further study is warranted to reveal the functional importance of COBLL1 on athletic performance in race horses. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015-06 /pmc/articles/PMC4412984/ /pubmed/25925064 http://dx.doi.org/10.5713/ajas.14.0722 Text en Copyright © 2015 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), wwhich permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Park, Jeong-Woong
Jang, Hyun-Jun
Shin, Sangsu
Cho, Hyun-Woo
Choi, Jae-Young
Kim, Nam-Young
Lee, Hak-Kyo
Do, Kyong-Tak
Song, Ki-Duk
Cho, Byung-Wook
Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title_full Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title_fullStr Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title_full_unstemmed Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title_short Molecular Analysis of Alternative Transcripts of the Equine Cordon-Bleu WH2 Repeat Protein-Like 1 (COBLL1) Gene
title_sort molecular analysis of alternative transcripts of the equine cordon-bleu wh2 repeat protein-like 1 (cobll1) gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412984/
https://www.ncbi.nlm.nih.gov/pubmed/25925064
http://dx.doi.org/10.5713/ajas.14.0722
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