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Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs
The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting aflatoxin B(1) (AFB(1)). AFB(1)-free corn samples supplemented with different levels of AFB(1) (5, 10, and 20 μg/kg) we...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413000/ https://www.ncbi.nlm.nih.gov/pubmed/25924961 http://dx.doi.org/10.5713/ajas.14.0868 |
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author | Sun, Dan-dan Gu, Xu Li, Jun-guo Yao, Ting Dong, Ying-chao |
author_facet | Sun, Dan-dan Gu, Xu Li, Jun-guo Yao, Ting Dong, Ying-chao |
author_sort | Sun, Dan-dan |
collection | PubMed |
description | The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting aflatoxin B(1) (AFB(1)). AFB(1)-free corn samples supplemented with different levels of AFB(1) (5, 10, and 20 μg/kg) were used as positive controls and 6 replicates of each control sample were tested to evaluate the accuracy and precision of these kits. In addition, we also evaluated the performance of these ELISA kits for AFB(1) in 30 feed samples, including corn, distillers dried grains with soluble, wheat samples, soybean meal, and poultry feed, which were verified by high performance liquid chromatography. Results showed that the coefficients of variation ranged from 1.18% to 16.22% in intra-plate and 2.85% to 18.04% in inter-plate for the determination of AFB(1). The half maximal inhibitory concentration for five kits ranged from 3.72 to 7.22 μg/kg. The quantitation limits of AFB(1) were all under the legal limit in China but somewhat inconsistent with kit instructions. Although the recovery rate of four of the five kits were either less than 90% or more than 110%, all these values were acceptable in practice. Two kits had high false positive rates (C and E). In conclusion, our results revealed that the qualities of five tested ELISA kits were significantly different. |
format | Online Article Text |
id | pubmed-4413000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-44130002015-05-01 Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs Sun, Dan-dan Gu, Xu Li, Jun-guo Yao, Ting Dong, Ying-chao Asian-Australas J Anim Sci Article The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting aflatoxin B(1) (AFB(1)). AFB(1)-free corn samples supplemented with different levels of AFB(1) (5, 10, and 20 μg/kg) were used as positive controls and 6 replicates of each control sample were tested to evaluate the accuracy and precision of these kits. In addition, we also evaluated the performance of these ELISA kits for AFB(1) in 30 feed samples, including corn, distillers dried grains with soluble, wheat samples, soybean meal, and poultry feed, which were verified by high performance liquid chromatography. Results showed that the coefficients of variation ranged from 1.18% to 16.22% in intra-plate and 2.85% to 18.04% in inter-plate for the determination of AFB(1). The half maximal inhibitory concentration for five kits ranged from 3.72 to 7.22 μg/kg. The quantitation limits of AFB(1) were all under the legal limit in China but somewhat inconsistent with kit instructions. Although the recovery rate of four of the five kits were either less than 90% or more than 110%, all these values were acceptable in practice. Two kits had high false positive rates (C and E). In conclusion, our results revealed that the qualities of five tested ELISA kits were significantly different. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015-05 /pmc/articles/PMC4413000/ /pubmed/25924961 http://dx.doi.org/10.5713/ajas.14.0868 Text en Copyright © 2015 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), wwhich permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Sun, Dan-dan Gu, Xu Li, Jun-guo Yao, Ting Dong, Ying-chao Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title | Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title_full | Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title_fullStr | Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title_full_unstemmed | Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title_short | Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B(1) in Feedstuffs |
title_sort | quality evaluation of five commercial enzyme linked immunosorbent assay kits for detecting aflatoxin b(1) in feedstuffs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413000/ https://www.ncbi.nlm.nih.gov/pubmed/25924961 http://dx.doi.org/10.5713/ajas.14.0868 |
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