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Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats
This study's aim was to determine the effect of hydroalcoholic extract of M. cauliflora (HEMC) on vascular tension and blood pressure in rats. In our in vitro studies using precontracted isolated aortas from rats, HEMC and acetylcholine (positive control) induced relaxation only in vessels with...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413038/ https://www.ncbi.nlm.nih.gov/pubmed/25960756 http://dx.doi.org/10.1155/2015/696135 |
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author | Lobo de Andrade, Daniela Medeiros Reis, Carolina de Fátima Castro, Patrícia Ferreira da Silva Borges, Leonardo Luiz Amaral, Nathalia Oda Torres, Ieda Maria Sapateiro Rezende, Stefani Garcia Gil, Eric de Souza Cardoso da Conceição, Edemilson Pedrino, Gustavo Rodrigues Lavorenti Rocha, Matheus |
author_facet | Lobo de Andrade, Daniela Medeiros Reis, Carolina de Fátima Castro, Patrícia Ferreira da Silva Borges, Leonardo Luiz Amaral, Nathalia Oda Torres, Ieda Maria Sapateiro Rezende, Stefani Garcia Gil, Eric de Souza Cardoso da Conceição, Edemilson Pedrino, Gustavo Rodrigues Lavorenti Rocha, Matheus |
author_sort | Lobo de Andrade, Daniela Medeiros |
collection | PubMed |
description | This study's aim was to determine the effect of hydroalcoholic extract of M. cauliflora (HEMC) on vascular tension and blood pressure in rats. In our in vitro studies using precontracted isolated aortas from rats, HEMC and acetylcholine (positive control) induced relaxation only in vessels with endothelium. Pretreatment with L-NAME (NO synthase inhibitor) or ODQ (soluble guanylyl cyclase (sGC) inhibitor) abolished the HEMC-induced relaxation. The treatment with MDL-12,330A (adenylyl cyclase (AC) inhibitor) or diclofenac (COX inhibitor) reduced HEMC-induced vasorelaxation. The blockade of muscarinic and β-adrenergic receptors (by atropine and propranolol, resp.) did not promote changes in HEMC-induced vasorelaxation. In our in vivo studies, catheters were inserted into the right femoral vein and artery of anesthetized rats for HEMC infusion and the measurement of blood pressure, heart rate, and aortic blood flow. The intravenous infusion of HEMC produced hypotension and increased aortic blood flow with no changes in heart rate. These findings showed that HEMC induces endothelium-dependent vascular relaxation and hypotension with no alteration in heart rate. The NO/sGC/cGMP pathway seems to be the main cellular route involved in the vascular responsiveness. |
format | Online Article Text |
id | pubmed-4413038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44130382015-05-10 Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats Lobo de Andrade, Daniela Medeiros Reis, Carolina de Fátima Castro, Patrícia Ferreira da Silva Borges, Leonardo Luiz Amaral, Nathalia Oda Torres, Ieda Maria Sapateiro Rezende, Stefani Garcia Gil, Eric de Souza Cardoso da Conceição, Edemilson Pedrino, Gustavo Rodrigues Lavorenti Rocha, Matheus Evid Based Complement Alternat Med Research Article This study's aim was to determine the effect of hydroalcoholic extract of M. cauliflora (HEMC) on vascular tension and blood pressure in rats. In our in vitro studies using precontracted isolated aortas from rats, HEMC and acetylcholine (positive control) induced relaxation only in vessels with endothelium. Pretreatment with L-NAME (NO synthase inhibitor) or ODQ (soluble guanylyl cyclase (sGC) inhibitor) abolished the HEMC-induced relaxation. The treatment with MDL-12,330A (adenylyl cyclase (AC) inhibitor) or diclofenac (COX inhibitor) reduced HEMC-induced vasorelaxation. The blockade of muscarinic and β-adrenergic receptors (by atropine and propranolol, resp.) did not promote changes in HEMC-induced vasorelaxation. In our in vivo studies, catheters were inserted into the right femoral vein and artery of anesthetized rats for HEMC infusion and the measurement of blood pressure, heart rate, and aortic blood flow. The intravenous infusion of HEMC produced hypotension and increased aortic blood flow with no changes in heart rate. These findings showed that HEMC induces endothelium-dependent vascular relaxation and hypotension with no alteration in heart rate. The NO/sGC/cGMP pathway seems to be the main cellular route involved in the vascular responsiveness. Hindawi Publishing Corporation 2015 2015-04-15 /pmc/articles/PMC4413038/ /pubmed/25960756 http://dx.doi.org/10.1155/2015/696135 Text en Copyright © 2015 Daniela Medeiros Lobo de Andrade et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lobo de Andrade, Daniela Medeiros Reis, Carolina de Fátima Castro, Patrícia Ferreira da Silva Borges, Leonardo Luiz Amaral, Nathalia Oda Torres, Ieda Maria Sapateiro Rezende, Stefani Garcia Gil, Eric de Souza Cardoso da Conceição, Edemilson Pedrino, Gustavo Rodrigues Lavorenti Rocha, Matheus Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title | Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title_full | Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title_fullStr | Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title_full_unstemmed | Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title_short | Vasorelaxant and Hypotensive Effects of Jaboticaba Fruit (Myrciaria cauliflora) Extract in Rats |
title_sort | vasorelaxant and hypotensive effects of jaboticaba fruit (myrciaria cauliflora) extract in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413038/ https://www.ncbi.nlm.nih.gov/pubmed/25960756 http://dx.doi.org/10.1155/2015/696135 |
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