Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies

The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at...

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Detalles Bibliográficos
Autores principales: Al-Kurdi, Zakieh I., Chowdhry, Babur Z., Leharne, Stephen A., Al Omari, Mahmoud M. H., Badwan, Adnan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413186/
https://www.ncbi.nlm.nih.gov/pubmed/25830681
http://dx.doi.org/10.3390/md13041765
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author Al-Kurdi, Zakieh I.
Chowdhry, Babur Z.
Leharne, Stephen A.
Al Omari, Mahmoud M. H.
Badwan, Adnan A.
author_facet Al-Kurdi, Zakieh I.
Chowdhry, Babur Z.
Leharne, Stephen A.
Al Omari, Mahmoud M. H.
Badwan, Adnan A.
author_sort Al-Kurdi, Zakieh I.
collection PubMed
description The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity.
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spelling pubmed-44131862015-05-07 Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies Al-Kurdi, Zakieh I. Chowdhry, Babur Z. Leharne, Stephen A. Al Omari, Mahmoud M. H. Badwan, Adnan A. Mar Drugs Article The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity. MDPI 2015-03-30 /pmc/articles/PMC4413186/ /pubmed/25830681 http://dx.doi.org/10.3390/md13041765 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Kurdi, Zakieh I.
Chowdhry, Babur Z.
Leharne, Stephen A.
Al Omari, Mahmoud M. H.
Badwan, Adnan A.
Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title_full Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title_fullStr Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title_full_unstemmed Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title_short Low Molecular Weight Chitosan–Insulin Polyelectrolyte Complex: Characterization and Stability Studies
title_sort low molecular weight chitosan–insulin polyelectrolyte complex: characterization and stability studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413186/
https://www.ncbi.nlm.nih.gov/pubmed/25830681
http://dx.doi.org/10.3390/md13041765
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