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Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ
OBJECTIVE: To determine whether super-activation of PPARγ can reprogram human myoblasts into brownlike adipocytes and to establish a new cell model for browning research. METHODS: To enhance the PPARγ signaling, we fused M3, the transactivation domain of MyoD, to PPARγ. PPARγ and M3-PPARγ-lentiviral...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413469/ https://www.ncbi.nlm.nih.gov/pubmed/25919922 http://dx.doi.org/10.1002/oby.21062 |
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author | Zhu, Yanbei Yang, Rongze McLenithan, John Yu, Daozhan Wang, Hong Wang, Yaping Singh, Devinder Olson, John Sztalryd, Carole Zhu, Dalong Gong, Da-Wei |
author_facet | Zhu, Yanbei Yang, Rongze McLenithan, John Yu, Daozhan Wang, Hong Wang, Yaping Singh, Devinder Olson, John Sztalryd, Carole Zhu, Dalong Gong, Da-Wei |
author_sort | Zhu, Yanbei |
collection | PubMed |
description | OBJECTIVE: To determine whether super-activation of PPARγ can reprogram human myoblasts into brownlike adipocytes and to establish a new cell model for browning research. METHODS: To enhance the PPARγ signaling, we fused M3, the transactivation domain of MyoD, to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. RESULTS: In transient transfection, M3-PPARγ has stronger constitutive activity than PPARγ reporter assay. Although both the transduction of PPARγ and M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ, compared to PPARγ, drastically induced the brown adipocyte markers of UCP1, CIDEA and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold. The gene expression levels of the browning makers in PPARγ-reprogrammed adipocytes are comparable to those of in vitro differentiated white adipocytes. CONCLUSIONS: We have found that super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and can be a new approach to derive brown-like adipocytes. |
format | Online Article Text |
id | pubmed-4413469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-44134692016-05-01 Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ Zhu, Yanbei Yang, Rongze McLenithan, John Yu, Daozhan Wang, Hong Wang, Yaping Singh, Devinder Olson, John Sztalryd, Carole Zhu, Dalong Gong, Da-Wei Obesity (Silver Spring) Article OBJECTIVE: To determine whether super-activation of PPARγ can reprogram human myoblasts into brownlike adipocytes and to establish a new cell model for browning research. METHODS: To enhance the PPARγ signaling, we fused M3, the transactivation domain of MyoD, to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. RESULTS: In transient transfection, M3-PPARγ has stronger constitutive activity than PPARγ reporter assay. Although both the transduction of PPARγ and M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ, compared to PPARγ, drastically induced the brown adipocyte markers of UCP1, CIDEA and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold. The gene expression levels of the browning makers in PPARγ-reprogrammed adipocytes are comparable to those of in vitro differentiated white adipocytes. CONCLUSIONS: We have found that super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and can be a new approach to derive brown-like adipocytes. 2015-05 /pmc/articles/PMC4413469/ /pubmed/25919922 http://dx.doi.org/10.1002/oby.21062 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Zhu, Yanbei Yang, Rongze McLenithan, John Yu, Daozhan Wang, Hong Wang, Yaping Singh, Devinder Olson, John Sztalryd, Carole Zhu, Dalong Gong, Da-Wei Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title | Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title_full | Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title_fullStr | Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title_full_unstemmed | Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title_short | Direct conversion of human myoblasts into brown-like adipocytes by engineered superactive PPARγ |
title_sort | direct conversion of human myoblasts into brown-like adipocytes by engineered superactive pparγ |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413469/ https://www.ncbi.nlm.nih.gov/pubmed/25919922 http://dx.doi.org/10.1002/oby.21062 |
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