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Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms

INTRODUCTION: For many tumors, the overexpression of the chemokine receptor CXCR4 is associated with increased malignancy and poor patient outcomes. However, comprehensive data for neuroendocrine neoplasms of the lung are still lacking. METHODS: CXCR4 expression was evaluated in a panel of bronchopu...

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Autores principales: Kaemmerer, Daniel, Reimann, Christiane, Specht, Elisa, Wirtz, Ralph M., Sayeg, Manal, Baum, Richard P., Schulz, Stefan, Lupp, Amelie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413658/
https://www.ncbi.nlm.nih.gov/pubmed/25671300
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author Kaemmerer, Daniel
Reimann, Christiane
Specht, Elisa
Wirtz, Ralph M.
Sayeg, Manal
Baum, Richard P.
Schulz, Stefan
Lupp, Amelie
author_facet Kaemmerer, Daniel
Reimann, Christiane
Specht, Elisa
Wirtz, Ralph M.
Sayeg, Manal
Baum, Richard P.
Schulz, Stefan
Lupp, Amelie
author_sort Kaemmerer, Daniel
collection PubMed
description INTRODUCTION: For many tumors, the overexpression of the chemokine receptor CXCR4 is associated with increased malignancy and poor patient outcomes. However, comprehensive data for neuroendocrine neoplasms of the lung are still lacking. METHODS: CXCR4 expression was evaluated in a panel of bronchopulmonary neuroendocrine neoplasms (BP-NEN) comprising typical carcinoids (n = 26), atypical carcinoids (n = 30), and small cell lung cancers (SCLC, n = 34). Samples were analyzed by immunohistochemistry using the novel monoclonal rabbit anti-human CXCR4 antibody UMB-2 and by qRT-PCR. The expression was correlated with clinical data and overall patient survival. RESULTS: CXCR4 was predominantly localized at the plasma membrane of the tumor cells. CXCR4 was expressed with a high intensity in almost all of the 30 SCLC samples. In contrast, it was detected infrequently and with low intensity in the typical carcinoid and atypical carcinoid samples. There was a significant correlation between the immunohistochemistry and qRT-PCR data. Additionally, there was a significant negative relationship between CXCR4 expression and overall survival. CONCLUSIONS: With increasing malignancy, BP-NEN clearly differ in the extent of CXCR4 expression. As in other tumor entities, CXCR4 overexpression significantly correlates with negative patient outcome. Due to its particular high expression rate in SCLC, CXCR4 may serve as a promising new target for diagnostic and pharmacological intervention as well as for peptide receptor-based radionuclide therapy.
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spelling pubmed-44136582015-05-08 Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms Kaemmerer, Daniel Reimann, Christiane Specht, Elisa Wirtz, Ralph M. Sayeg, Manal Baum, Richard P. Schulz, Stefan Lupp, Amelie Oncotarget Research Paper INTRODUCTION: For many tumors, the overexpression of the chemokine receptor CXCR4 is associated with increased malignancy and poor patient outcomes. However, comprehensive data for neuroendocrine neoplasms of the lung are still lacking. METHODS: CXCR4 expression was evaluated in a panel of bronchopulmonary neuroendocrine neoplasms (BP-NEN) comprising typical carcinoids (n = 26), atypical carcinoids (n = 30), and small cell lung cancers (SCLC, n = 34). Samples were analyzed by immunohistochemistry using the novel monoclonal rabbit anti-human CXCR4 antibody UMB-2 and by qRT-PCR. The expression was correlated with clinical data and overall patient survival. RESULTS: CXCR4 was predominantly localized at the plasma membrane of the tumor cells. CXCR4 was expressed with a high intensity in almost all of the 30 SCLC samples. In contrast, it was detected infrequently and with low intensity in the typical carcinoid and atypical carcinoid samples. There was a significant correlation between the immunohistochemistry and qRT-PCR data. Additionally, there was a significant negative relationship between CXCR4 expression and overall survival. CONCLUSIONS: With increasing malignancy, BP-NEN clearly differ in the extent of CXCR4 expression. As in other tumor entities, CXCR4 overexpression significantly correlates with negative patient outcome. Due to its particular high expression rate in SCLC, CXCR4 may serve as a promising new target for diagnostic and pharmacological intervention as well as for peptide receptor-based radionuclide therapy. Impact Journals LLC 2014-12-30 /pmc/articles/PMC4413658/ /pubmed/25671300 Text en Copyright: © 2015 Kaemmerer et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Kaemmerer, Daniel
Reimann, Christiane
Specht, Elisa
Wirtz, Ralph M.
Sayeg, Manal
Baum, Richard P.
Schulz, Stefan
Lupp, Amelie
Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title_full Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title_fullStr Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title_full_unstemmed Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title_short Differential expression and prognostic value of the chemokine receptor CXCR4 in bronchopulmonary neuroendocrine neoplasms
title_sort differential expression and prognostic value of the chemokine receptor cxcr4 in bronchopulmonary neuroendocrine neoplasms
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413658/
https://www.ncbi.nlm.nih.gov/pubmed/25671300
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