Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9

CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high expression and sophisticated tools such as co...

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Detalles Bibliográficos
Autores principales: Rojas-Fernandez, Alejandro, Herhaus, Lina, Macartney, Thomas, Lachaud, Christophe, Hay, Ronald T., Sapkota, Gopal P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413877/
https://www.ncbi.nlm.nih.gov/pubmed/25922883
http://dx.doi.org/10.1038/srep09811

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413877/
https://www.ncbi.nlm.nih.gov/pubmed/25922883
http://dx.doi.org/10.1038/srep09811

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