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Cytokine Expression of Microscopic Colitis Including Interleukin-17

BACKGROUND/AIMS: Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis....

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Detalles Bibliográficos
Autores principales: Park, Eunkyoung, Park, Young Sook, Park, Dae Rim, Jung, Sung Ae, Han, Dong Soo, Jang, Byung Ik, Kim, Young Ho, Kim, Won Ho, Jo, Yun ju, Lee, Ki Ho, Lee, Won Mi, Kim, Eun Kyung, Koo, Hae Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Gut and Liver 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413972/
https://www.ncbi.nlm.nih.gov/pubmed/25071069
http://dx.doi.org/10.5009/gnl13439
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author Park, Eunkyoung
Park, Young Sook
Park, Dae Rim
Jung, Sung Ae
Han, Dong Soo
Jang, Byung Ik
Kim, Young Ho
Kim, Won Ho
Jo, Yun ju
Lee, Ki Ho
Lee, Won Mi
Kim, Eun Kyung
Koo, Hae Soo
author_facet Park, Eunkyoung
Park, Young Sook
Park, Dae Rim
Jung, Sung Ae
Han, Dong Soo
Jang, Byung Ik
Kim, Young Ho
Kim, Won Ho
Jo, Yun ju
Lee, Ki Ho
Lee, Won Mi
Kim, Eun Kyung
Koo, Hae Soo
author_sort Park, Eunkyoung
collection PubMed
description BACKGROUND/AIMS: Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis. METHODS: This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six patients with functional diarrhea but normal pathology. We performed an immunohistochemical analysis of the colonic mucosal biopsies to assess the expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, interferon-γ, inducible nitric oxide synthase, and tumor necrosis factor-α. We compared the quantity score of immunohistochemical staining among the groups. RESULTS: The microscopic colitis group showed significantly higher expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, and interferon-γ compared with the control group. Cytokine expression was similar between collagenous colitis and lymphocytic colitis. However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis. CONCLUSIONS: Proinflammatory cytokines, including interleukin-17 and interferon-γ, are highly expressed in microscopic colitis. The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis. This study is the first on interleukin-17 expression in microscopic colitis patients.
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spelling pubmed-44139722015-05-06 Cytokine Expression of Microscopic Colitis Including Interleukin-17 Park, Eunkyoung Park, Young Sook Park, Dae Rim Jung, Sung Ae Han, Dong Soo Jang, Byung Ik Kim, Young Ho Kim, Won Ho Jo, Yun ju Lee, Ki Ho Lee, Won Mi Kim, Eun Kyung Koo, Hae Soo Gut Liver Original Article BACKGROUND/AIMS: Microscopic colitis is characterized by chronic watery diarrhea with specific pathological changes that can be diagnosed by microscopic examination. We performed immunohistochemical analysis of proinflammatory cytokines to investigate the pathogenic mechanism of microscopic colitis. METHODS: This study consisted of six patients with lymphocytic colitis, six patients with collagenous colitis, and six patients with functional diarrhea but normal pathology. We performed an immunohistochemical analysis of the colonic mucosal biopsies to assess the expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, interferon-γ, inducible nitric oxide synthase, and tumor necrosis factor-α. We compared the quantity score of immunohistochemical staining among the groups. RESULTS: The microscopic colitis group showed significantly higher expression of cyclo-oxygenase-2, interleukin-17, nuclear factor-κB, and interferon-γ compared with the control group. Cytokine expression was similar between collagenous colitis and lymphocytic colitis. However, the expression of cyclo-oxygenase-2 was higher in collagenous colitis. CONCLUSIONS: Proinflammatory cytokines, including interleukin-17 and interferon-γ, are highly expressed in microscopic colitis. The expression of cyclo-oxygenase-2 was higher in collagenous colitis than in lymphocytic colitis. This study is the first on interleukin-17 expression in microscopic colitis patients. Gut and Liver 2015-05 2014-07-25 /pmc/articles/PMC4413972/ /pubmed/25071069 http://dx.doi.org/10.5009/gnl13439 Text en Copyright © 2015 by The Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, Korean College of Helicobacter and Upper Gastrointestinal Research, Korean Association for the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, Korean Pancreatobiliary Association, and Korean Society of Gastrointestinal Cancer. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Eunkyoung
Park, Young Sook
Park, Dae Rim
Jung, Sung Ae
Han, Dong Soo
Jang, Byung Ik
Kim, Young Ho
Kim, Won Ho
Jo, Yun ju
Lee, Ki Ho
Lee, Won Mi
Kim, Eun Kyung
Koo, Hae Soo
Cytokine Expression of Microscopic Colitis Including Interleukin-17
title Cytokine Expression of Microscopic Colitis Including Interleukin-17
title_full Cytokine Expression of Microscopic Colitis Including Interleukin-17
title_fullStr Cytokine Expression of Microscopic Colitis Including Interleukin-17
title_full_unstemmed Cytokine Expression of Microscopic Colitis Including Interleukin-17
title_short Cytokine Expression of Microscopic Colitis Including Interleukin-17
title_sort cytokine expression of microscopic colitis including interleukin-17
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413972/
https://www.ncbi.nlm.nih.gov/pubmed/25071069
http://dx.doi.org/10.5009/gnl13439
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