Cargando…
Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer
Zinc-finger protein 545 (ZNF545) was identified as a gastric tumour suppressor and potentially independent prognostic factor. At the present study, we found that lower expression of ZNF545 was specific in gastric cancer (GC) tissues, and the inconsistently methylated levels of ZNF545 promoter were i...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414205/ https://www.ncbi.nlm.nih.gov/pubmed/25714013 |
_version_ | 1782368897651965952 |
---|---|
author | Deng, Jingyu Liang, Han Ying, Guoguang Dong, Qiuping Zhang, Rupeng Yu, Jun Fan, Daiming Hao, Xishan |
author_facet | Deng, Jingyu Liang, Han Ying, Guoguang Dong, Qiuping Zhang, Rupeng Yu, Jun Fan, Daiming Hao, Xishan |
author_sort | Deng, Jingyu |
collection | PubMed |
description | Zinc-finger protein 545 (ZNF545) was identified as a gastric tumour suppressor and potentially independent prognostic factor. At the present study, we found that lower expression of ZNF545 was specific in gastric cancer (GC) tissues, and the inconsistently methylated levels of ZNF545 promoter were identified in the gastric cancer tissues. In the methylation-specific PCR (MSP) analysis cohort, we found that GC patients with hypermethylated ZNF545 promoter exhibited significantly shorter median OS than those with unmethylated ZNF545 promoter and those with hypomethylated ZNF545 promoter. In the other cohort, we also demonstrated that GC patients with three or more methylated CpG sites in the ZNF545 promoter were significantly associated with poor survival by using the bisulphite gene sequencing (BGS). The methylated degrees of five CpG sites (−232, −214, −176, −144 and −116) could also provide distinct survival discrimination of patients with GC. These findings indicated that the methylated CpG sites of the ZNF545 promoter could be used for the clinical prediction of the prognosis of GC. |
format | Online Article Text |
id | pubmed-4414205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-44142052015-05-08 Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer Deng, Jingyu Liang, Han Ying, Guoguang Dong, Qiuping Zhang, Rupeng Yu, Jun Fan, Daiming Hao, Xishan Oncotarget Clinical Research Paper Zinc-finger protein 545 (ZNF545) was identified as a gastric tumour suppressor and potentially independent prognostic factor. At the present study, we found that lower expression of ZNF545 was specific in gastric cancer (GC) tissues, and the inconsistently methylated levels of ZNF545 promoter were identified in the gastric cancer tissues. In the methylation-specific PCR (MSP) analysis cohort, we found that GC patients with hypermethylated ZNF545 promoter exhibited significantly shorter median OS than those with unmethylated ZNF545 promoter and those with hypomethylated ZNF545 promoter. In the other cohort, we also demonstrated that GC patients with three or more methylated CpG sites in the ZNF545 promoter were significantly associated with poor survival by using the bisulphite gene sequencing (BGS). The methylated degrees of five CpG sites (−232, −214, −176, −144 and −116) could also provide distinct survival discrimination of patients with GC. These findings indicated that the methylated CpG sites of the ZNF545 promoter could be used for the clinical prediction of the prognosis of GC. Impact Journals LLC 2015-02-26 /pmc/articles/PMC4414205/ /pubmed/25714013 Text en Copyright: © 2015 Deng et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Clinical Research Paper Deng, Jingyu Liang, Han Ying, Guoguang Dong, Qiuping Zhang, Rupeng Yu, Jun Fan, Daiming Hao, Xishan Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title | Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title_full | Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title_fullStr | Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title_full_unstemmed | Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title_short | Poor survival is associated with the methylated degree of zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer |
title_sort | poor survival is associated with the methylated degree of zinc-finger protein 545 (znf545) dna promoter in gastric cancer |
topic | Clinical Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414205/ https://www.ncbi.nlm.nih.gov/pubmed/25714013 |
work_keys_str_mv | AT dengjingyu poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT lianghan poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT yingguoguang poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT dongqiuping poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT zhangrupeng poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT yujun poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT fandaiming poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer AT haoxishan poorsurvivalisassociatedwiththemethylateddegreeofzincfingerprotein545znf545dnapromoteringastriccancer |