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X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein
Phanta is a reversibly photoswitching chromoprotein (Φ(F), 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (Φ(F), 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in comb...
Autores principales: | Don Paul, Craig, Traore, Daouda A. K., Olsen, Seth, Devenish, Rodney J., Close, Devin W., Bell, Toby D. M., Bradbury, Andrew, Wilce, Matthew C. J., Prescott, Mark |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414407/ https://www.ncbi.nlm.nih.gov/pubmed/25923520 http://dx.doi.org/10.1371/journal.pone.0123338 |
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