High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile

Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping...

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Detalles Bibliográficos
Autores principales: Rizzardi, Kristina, Åkerlund, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414534/
https://www.ncbi.nlm.nih.gov/pubmed/25923527
http://dx.doi.org/10.1371/journal.pone.0122457
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author Rizzardi, Kristina
Åkerlund, Thomas
author_facet Rizzardi, Kristina
Åkerlund, Thomas
author_sort Rizzardi, Kristina
collection PubMed
description Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping, the method is extremely fast and simple, and supports for cost-effective screening of isolates during outbreak situations.
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spelling pubmed-44145342015-05-07 High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile Rizzardi, Kristina Åkerlund, Thomas PLoS One Research Article Clostridium difficile strains were typed by a newly developed MALDI-TOF method, high molecular weight typing, and compared to PCR ribotyping. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Although less discriminatory than PCR ribotyping, the method is extremely fast and simple, and supports for cost-effective screening of isolates during outbreak situations. Public Library of Science 2015-04-29 /pmc/articles/PMC4414534/ /pubmed/25923527 http://dx.doi.org/10.1371/journal.pone.0122457 Text en © 2015 Rizzardi, Åkerlund http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rizzardi, Kristina
Åkerlund, Thomas
High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title_full High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title_fullStr High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title_full_unstemmed High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title_short High Molecular Weight Typing with MALDI-TOF MS - A Novel Method for Rapid Typing of Clostridium difficile
title_sort high molecular weight typing with maldi-tof ms - a novel method for rapid typing of clostridium difficile
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414534/
https://www.ncbi.nlm.nih.gov/pubmed/25923527
http://dx.doi.org/10.1371/journal.pone.0122457
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