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A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles

The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report...

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Autores principales: Garai, Ellis, Sensarn, Steven, Zavaleta, Cristina L., Loewke, Nathan O., Rogalla, Stephan, Mandella, Michael J., Felt, Stephen A., Friedland, Shai, Liu, Jonathan T. C., Gambhir, Sanjiv S., Contag, Christopher H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414592/
https://www.ncbi.nlm.nih.gov/pubmed/25923788
http://dx.doi.org/10.1371/journal.pone.0123185
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author Garai, Ellis
Sensarn, Steven
Zavaleta, Cristina L.
Loewke, Nathan O.
Rogalla, Stephan
Mandella, Michael J.
Felt, Stephen A.
Friedland, Shai
Liu, Jonathan T. C.
Gambhir, Sanjiv S.
Contag, Christopher H.
author_facet Garai, Ellis
Sensarn, Steven
Zavaleta, Cristina L.
Loewke, Nathan O.
Rogalla, Stephan
Mandella, Michael J.
Felt, Stephen A.
Friedland, Shai
Liu, Jonathan T. C.
Gambhir, Sanjiv S.
Contag, Christopher H.
author_sort Garai, Ellis
collection PubMed
description The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e.g., colon and esophagus) and produces quantitative images of the relative concentrations of SERS NPs that are present. Human and swine studies have demonstrated the speed and simplicity of this technique. This approach also offers unparalleled multiplexing capabilities by simultaneously detecting the unique spectral fingerprints of multiple SERS NPs. Therefore, this new screening strategy has the potential to improve diagnosis and to guide therapy by enabling sensitive quantitative molecular detection of small and otherwise hard-to-detect lesions in the context of white-light endoscopy.
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spelling pubmed-44145922015-05-07 A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles Garai, Ellis Sensarn, Steven Zavaleta, Cristina L. Loewke, Nathan O. Rogalla, Stephan Mandella, Michael J. Felt, Stephen A. Friedland, Shai Liu, Jonathan T. C. Gambhir, Sanjiv S. Contag, Christopher H. PLoS One Research Article The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e.g., colon and esophagus) and produces quantitative images of the relative concentrations of SERS NPs that are present. Human and swine studies have demonstrated the speed and simplicity of this technique. This approach also offers unparalleled multiplexing capabilities by simultaneously detecting the unique spectral fingerprints of multiple SERS NPs. Therefore, this new screening strategy has the potential to improve diagnosis and to guide therapy by enabling sensitive quantitative molecular detection of small and otherwise hard-to-detect lesions in the context of white-light endoscopy. Public Library of Science 2015-04-29 /pmc/articles/PMC4414592/ /pubmed/25923788 http://dx.doi.org/10.1371/journal.pone.0123185 Text en © 2015 Garai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Garai, Ellis
Sensarn, Steven
Zavaleta, Cristina L.
Loewke, Nathan O.
Rogalla, Stephan
Mandella, Michael J.
Felt, Stephen A.
Friedland, Shai
Liu, Jonathan T. C.
Gambhir, Sanjiv S.
Contag, Christopher H.
A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title_full A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title_fullStr A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title_full_unstemmed A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title_short A Real-Time Clinical Endoscopic System for Intraluminal, Multiplexed Imaging of Surface-Enhanced Raman Scattering Nanoparticles
title_sort real-time clinical endoscopic system for intraluminal, multiplexed imaging of surface-enhanced raman scattering nanoparticles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414592/
https://www.ncbi.nlm.nih.gov/pubmed/25923788
http://dx.doi.org/10.1371/journal.pone.0123185
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