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Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans

OBJECTIVE: Investigate the effects of obesity and high fat diet (HFD) exposure on fatty acid oxidation and TCA cycle intermediates and amino acids in skeletal muscle to better characterize energy metabolism. DESIGN AND METHODS: Plasma and skeletal muscle metabolomic profiles were measured from lean...

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Autores principales: Baker, Peter R., Boyle, Kristen E., Koves, Timothy R., Ilkayeva, Olga R., Muoio, Deborah M., Houmard, Joseph A., Friedman, Jacob E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414721/
https://www.ncbi.nlm.nih.gov/pubmed/25864501
http://dx.doi.org/10.1002/oby.21046
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author Baker, Peter R.
Boyle, Kristen E.
Koves, Timothy R.
Ilkayeva, Olga R.
Muoio, Deborah M.
Houmard, Joseph A.
Friedman, Jacob E.
author_facet Baker, Peter R.
Boyle, Kristen E.
Koves, Timothy R.
Ilkayeva, Olga R.
Muoio, Deborah M.
Houmard, Joseph A.
Friedman, Jacob E.
author_sort Baker, Peter R.
collection PubMed
description OBJECTIVE: Investigate the effects of obesity and high fat diet (HFD) exposure on fatty acid oxidation and TCA cycle intermediates and amino acids in skeletal muscle to better characterize energy metabolism. DESIGN AND METHODS: Plasma and skeletal muscle metabolomic profiles were measured from lean and obese males before and after a 5 day HFD in the 4h post-prandial condition. RESULTS: At both time points, plasma short-chain acylcarnitine species (SCAC) were higher in the obese subjects, while the amino acids glycine, histidine, methionine, and citrulline were lower in skeletal muscle of obese subjects. Skeletal muscle medium-chain acylcarnitines (MCAC) C6, C8, C10:2, C10:1, C10, and C12:1 increased in obese subjects, but decreased in lean subjects, from Pre- to Post-HFD. Plasma content of C10:1 was also decreased in lean, but increased in the obese subjects from Pre- to Post-HFD. CD36 increased from Pre- to Post-HFD in obese but not lean subjects. CONCLUSIONS: Lower skeletal muscle amino acid content and accumulation of plasma SCAC in obese subjects could reflect increased anaplerosis for TCA cycle intermediates, while accumulation of MCAC suggests limitations in β-oxidation. These measures may be important markers of or contributors to dysregulated metabolism observed in skeletal muscle of obese humans.
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spelling pubmed-44147212016-05-01 Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans Baker, Peter R. Boyle, Kristen E. Koves, Timothy R. Ilkayeva, Olga R. Muoio, Deborah M. Houmard, Joseph A. Friedman, Jacob E. Obesity (Silver Spring) Article OBJECTIVE: Investigate the effects of obesity and high fat diet (HFD) exposure on fatty acid oxidation and TCA cycle intermediates and amino acids in skeletal muscle to better characterize energy metabolism. DESIGN AND METHODS: Plasma and skeletal muscle metabolomic profiles were measured from lean and obese males before and after a 5 day HFD in the 4h post-prandial condition. RESULTS: At both time points, plasma short-chain acylcarnitine species (SCAC) were higher in the obese subjects, while the amino acids glycine, histidine, methionine, and citrulline were lower in skeletal muscle of obese subjects. Skeletal muscle medium-chain acylcarnitines (MCAC) C6, C8, C10:2, C10:1, C10, and C12:1 increased in obese subjects, but decreased in lean subjects, from Pre- to Post-HFD. Plasma content of C10:1 was also decreased in lean, but increased in the obese subjects from Pre- to Post-HFD. CD36 increased from Pre- to Post-HFD in obese but not lean subjects. CONCLUSIONS: Lower skeletal muscle amino acid content and accumulation of plasma SCAC in obese subjects could reflect increased anaplerosis for TCA cycle intermediates, while accumulation of MCAC suggests limitations in β-oxidation. These measures may be important markers of or contributors to dysregulated metabolism observed in skeletal muscle of obese humans. 2015-04-10 2015-05 /pmc/articles/PMC4414721/ /pubmed/25864501 http://dx.doi.org/10.1002/oby.21046 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Baker, Peter R.
Boyle, Kristen E.
Koves, Timothy R.
Ilkayeva, Olga R.
Muoio, Deborah M.
Houmard, Joseph A.
Friedman, Jacob E.
Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title_full Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title_fullStr Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title_full_unstemmed Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title_short Metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
title_sort metabolomic analysis reveals decreased skeletal muscle amino acid content and altered fatty acid handling in obese humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414721/
https://www.ncbi.nlm.nih.gov/pubmed/25864501
http://dx.doi.org/10.1002/oby.21046
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