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In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines
OBJECTIVE(S): Chlorpyrifos (CP) is a broad-spectrum organophosphorus pesticide used extensively in agricultural and domestic pest control, accounting for 50% of the global insecticidal use. In the present study, protective effects of two selenium-enriched strong antioxidative medicines IMOD and Angi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414995/ https://www.ncbi.nlm.nih.gov/pubmed/25945242 |
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author | Navaei-Nigjeh, Mona Asadi, Hamidreza Baeeri, Maryam Pedram, Sahar Rezvanfar, Mohammad Amin Mohammadirad, Azadeh Abdollahi, Mohammad |
author_facet | Navaei-Nigjeh, Mona Asadi, Hamidreza Baeeri, Maryam Pedram, Sahar Rezvanfar, Mohammad Amin Mohammadirad, Azadeh Abdollahi, Mohammad |
author_sort | Navaei-Nigjeh, Mona |
collection | PubMed |
description | OBJECTIVE(S): Chlorpyrifos (CP) is a broad-spectrum organophosphorus pesticide used extensively in agricultural and domestic pest control, accounting for 50% of the global insecticidal use. In the present study, protective effects of two selenium-enriched strong antioxidative medicines IMOD and Angipars were examined in human lymphocytes treated with CP in vitro. MATERIALS AND METHODS: Isolated lymphocytes were exposed to 12 µg/ml CP either alone or in combination with effective doses (ED(50)) of IMOD (0.2 µg/ml) and Angipars (1 µg/ml). After 3 days incubation, the viability and oxidative stress markers including cellular lipid peroxidation (LPO), myeloperoxidase (MPO), total thiol molecules (TTM), and total antioxidant power (TAP) were evaluated. Also, the levels of tumor necrosis factor-α (TNF-α), as inflammatory index along with acetylcholinesterase (AChE) activity and cell apoptosis were assessed by flow cytometry. RESULTS: Results indicated that effective doses of IMOD and Angipars reduced CP-exposed lymphocyte mortality rate along with oxidative stress. Both agents restored CP-induced elevation of TNF-α and protected the lymphocytes from CP-induced apoptosis and necrosis. CONCLUSION: Overall, results confirm that IMOD and Angipars reduce the toxic effects associated with CP through free radical scavenging and protection from apoptosis and necrosis. |
format | Online Article Text |
id | pubmed-4414995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-44149952015-05-05 In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines Navaei-Nigjeh, Mona Asadi, Hamidreza Baeeri, Maryam Pedram, Sahar Rezvanfar, Mohammad Amin Mohammadirad, Azadeh Abdollahi, Mohammad Iran J Basic Med Sci Original Article OBJECTIVE(S): Chlorpyrifos (CP) is a broad-spectrum organophosphorus pesticide used extensively in agricultural and domestic pest control, accounting for 50% of the global insecticidal use. In the present study, protective effects of two selenium-enriched strong antioxidative medicines IMOD and Angipars were examined in human lymphocytes treated with CP in vitro. MATERIALS AND METHODS: Isolated lymphocytes were exposed to 12 µg/ml CP either alone or in combination with effective doses (ED(50)) of IMOD (0.2 µg/ml) and Angipars (1 µg/ml). After 3 days incubation, the viability and oxidative stress markers including cellular lipid peroxidation (LPO), myeloperoxidase (MPO), total thiol molecules (TTM), and total antioxidant power (TAP) were evaluated. Also, the levels of tumor necrosis factor-α (TNF-α), as inflammatory index along with acetylcholinesterase (AChE) activity and cell apoptosis were assessed by flow cytometry. RESULTS: Results indicated that effective doses of IMOD and Angipars reduced CP-exposed lymphocyte mortality rate along with oxidative stress. Both agents restored CP-induced elevation of TNF-α and protected the lymphocytes from CP-induced apoptosis and necrosis. CONCLUSION: Overall, results confirm that IMOD and Angipars reduce the toxic effects associated with CP through free radical scavenging and protection from apoptosis and necrosis. Mashhad University of Medical Sciences 2015-03 /pmc/articles/PMC4414995/ /pubmed/25945242 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Navaei-Nigjeh, Mona Asadi, Hamidreza Baeeri, Maryam Pedram, Sahar Rezvanfar, Mohammad Amin Mohammadirad, Azadeh Abdollahi, Mohammad In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title | In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title_full | In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title_fullStr | In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title_full_unstemmed | In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title_short | In vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
title_sort | in vitro protection of human lymphocytes from toxic effects of chlorpyrifos by selenium-enriched medicines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414995/ https://www.ncbi.nlm.nih.gov/pubmed/25945242 |
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