Hapten-directed spontaneous disulfide shuffling: a universal technology for site-directed covalent coupling of payloads to antibodies

Humanized hapten-binding IgGs were designed with an accessible cysteine close to their binding pockets, for specific covalent payload attachment. Individual analyses of known structures of digoxigenin (Dig)- and fluorescein (Fluo) binding antibodies and a new structure of a biotin (Biot)-binder, rev...

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Detalles Bibliográficos
Autores principales: Dengl, Stefan, Hoffmann, Eike, Grote, Michael, Wagner, Cornelia, Mundigl, Olaf, Georges, Guy, Thorey, Irmgard, Stubenrauch, Kay-Gunnar, Bujotzek, Alexander, Josel, Hans-Peter, Dziadek, Sebastian, Benz, Joerg, Brinkmann, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Federation of American Societies for Experimental Biology 2015
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415024/
https://www.ncbi.nlm.nih.gov/pubmed/25670234
http://dx.doi.org/10.1096/fj.14-263665

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