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Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio

Larval zebrafish was subjected to a methodological exploration of the gastrointestinal microbiota and transcriptome. Assessed was the impact of two dietary inclusion levels of a novel protein meal (NPM) of animal origin (ragworm Nereis virens) on the gastrointestinal tract (GIT). Microbial developme...

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Autores principales: Rurangwa, Eugene, Sipkema, Detmer, Kals, Jeroen, ter Veld, Menno, Forlenza, Maria, Bacanu, Gianina M., Smidt, Hauke, Palstra, Arjan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415425/
https://www.ncbi.nlm.nih.gov/pubmed/25983694
http://dx.doi.org/10.3389/fphys.2015.00133
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author Rurangwa, Eugene
Sipkema, Detmer
Kals, Jeroen
ter Veld, Menno
Forlenza, Maria
Bacanu, Gianina M.
Smidt, Hauke
Palstra, Arjan P.
author_facet Rurangwa, Eugene
Sipkema, Detmer
Kals, Jeroen
ter Veld, Menno
Forlenza, Maria
Bacanu, Gianina M.
Smidt, Hauke
Palstra, Arjan P.
author_sort Rurangwa, Eugene
collection PubMed
description Larval zebrafish was subjected to a methodological exploration of the gastrointestinal microbiota and transcriptome. Assessed was the impact of two dietary inclusion levels of a novel protein meal (NPM) of animal origin (ragworm Nereis virens) on the gastrointestinal tract (GIT). Microbial development was assessed over the first 21 days post egg fertilization (dpf) through 16S rRNA gene-based microbial composition profiling by pyrosequencing. Differentially expressed genes in the GIT were demonstrated at 21 dpf by whole transcriptome sequencing (mRNAseq). Larval zebrafish showed rapid temporal changes in microbial colonization but domination occurred by one to three bacterial species generally belonging to Proteobacteria and Firmicutes. The high iron content of NPM may have led to an increased relative abundance of bacteria that were related to potential pathogens and bacteria with an increased iron metabolism. Functional classification of the 328 differentially expressed genes indicated that the GIT of larvae fed at higher NPM level was more active in transmembrane ion transport and protein synthesis. mRNAseq analysis did not reveal a major activation of genes involved in the immune response or indicating differences in iron uptake and homeostasis in zebrafish fed at the high inclusion level of NPM.
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spelling pubmed-44154252015-05-15 Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio Rurangwa, Eugene Sipkema, Detmer Kals, Jeroen ter Veld, Menno Forlenza, Maria Bacanu, Gianina M. Smidt, Hauke Palstra, Arjan P. Front Physiol Physiology Larval zebrafish was subjected to a methodological exploration of the gastrointestinal microbiota and transcriptome. Assessed was the impact of two dietary inclusion levels of a novel protein meal (NPM) of animal origin (ragworm Nereis virens) on the gastrointestinal tract (GIT). Microbial development was assessed over the first 21 days post egg fertilization (dpf) through 16S rRNA gene-based microbial composition profiling by pyrosequencing. Differentially expressed genes in the GIT were demonstrated at 21 dpf by whole transcriptome sequencing (mRNAseq). Larval zebrafish showed rapid temporal changes in microbial colonization but domination occurred by one to three bacterial species generally belonging to Proteobacteria and Firmicutes. The high iron content of NPM may have led to an increased relative abundance of bacteria that were related to potential pathogens and bacteria with an increased iron metabolism. Functional classification of the 328 differentially expressed genes indicated that the GIT of larvae fed at higher NPM level was more active in transmembrane ion transport and protein synthesis. mRNAseq analysis did not reveal a major activation of genes involved in the immune response or indicating differences in iron uptake and homeostasis in zebrafish fed at the high inclusion level of NPM. Frontiers Media S.A. 2015-04-30 /pmc/articles/PMC4415425/ /pubmed/25983694 http://dx.doi.org/10.3389/fphys.2015.00133 Text en Copyright © 2015 Rurangwa, Sipkema, Kals, ter Veld, Forlenza, Bacanu, Smidt and Palstra. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Rurangwa, Eugene
Sipkema, Detmer
Kals, Jeroen
ter Veld, Menno
Forlenza, Maria
Bacanu, Gianina M.
Smidt, Hauke
Palstra, Arjan P.
Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title_full Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title_fullStr Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title_full_unstemmed Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title_short Impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish Danio rerio
title_sort impact of a novel protein meal on the gastrointestinal microbiota and the host transcriptome of larval zebrafish danio rerio
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415425/
https://www.ncbi.nlm.nih.gov/pubmed/25983694
http://dx.doi.org/10.3389/fphys.2015.00133
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