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Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes
PURPOSE: The etiology of keratoconus (KC) and the factors governing its progression are not well understood. It has been suggested that this disease might be caused by biochemical alterations in the cornea; changes in the expression profiles of human aqueous humor (hAH) proteins have been observed i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415580/ https://www.ncbi.nlm.nih.gov/pubmed/25999673 |
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author | Soria, Javier Villarrubia, Alberto Merayo-Lloves, Jesús Elortza, Félix Azkargorta, Mikel Alvarez de Toledo, Juan Rodriguez-Agirretxe, Iñaki Suarez, Tatiana Acera, Arantxa |
author_facet | Soria, Javier Villarrubia, Alberto Merayo-Lloves, Jesús Elortza, Félix Azkargorta, Mikel Alvarez de Toledo, Juan Rodriguez-Agirretxe, Iñaki Suarez, Tatiana Acera, Arantxa |
author_sort | Soria, Javier |
collection | PubMed |
description | PURPOSE: The etiology of keratoconus (KC) and the factors governing its progression are not well understood. It has been suggested that this disease might be caused by biochemical alterations in the cornea; changes in the expression profiles of human aqueous humor (hAH) proteins have been observed in some diseases. To gain a new insight into the molecular mechanisms of KC pathology, we examined the hAH proteomes of those in the advanced stages of this disease. We used a high-throughput mass spectrometry approach to compare hAH protein expression in patients with KC and in control subjects. METHODS: Aqueous humor samples were acquired from five keratoconus patients during keratoplasty surgery and from five myopic control subjects during phakic intraocular lens implantation. Quantitative mass spectrometry analysis using spectral counting was performed to determine the relative amounts of hAH proteins in the samples from KC patients and control individuals. RESULTS: All KC patients included in the study presented severe keratoconus (K2 >52 D), and slit-lamp examination revealed microfolds in Descemet’s membrane, without clinical signs of hydrops. We found significant differences between the expression levels of 16 proteins in the two groups. In KC samples, seven proteins were overexpressed and nine were underexpressed in comparison with the control group. Gene ontology analysis revealed that these deregulated proteins are implicated in several biologic processes, such as the regulation of proteolysis, responses to hypoxia, and responses to hydrogen peroxide, among others. CONCLUSIONS: The protein expression profiles in hAH from KC patients and myopic control subjects differ significantly. This result suggests that some components of the hAH proteome are involved in this disease. Further in-depth analysis of the hAH proteome should provide a better understanding of the mechanisms governing the pathophysiology of KC. |
format | Online Article Text |
id | pubmed-4415580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-44155802015-05-21 Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes Soria, Javier Villarrubia, Alberto Merayo-Lloves, Jesús Elortza, Félix Azkargorta, Mikel Alvarez de Toledo, Juan Rodriguez-Agirretxe, Iñaki Suarez, Tatiana Acera, Arantxa Mol Vis Research Article PURPOSE: The etiology of keratoconus (KC) and the factors governing its progression are not well understood. It has been suggested that this disease might be caused by biochemical alterations in the cornea; changes in the expression profiles of human aqueous humor (hAH) proteins have been observed in some diseases. To gain a new insight into the molecular mechanisms of KC pathology, we examined the hAH proteomes of those in the advanced stages of this disease. We used a high-throughput mass spectrometry approach to compare hAH protein expression in patients with KC and in control subjects. METHODS: Aqueous humor samples were acquired from five keratoconus patients during keratoplasty surgery and from five myopic control subjects during phakic intraocular lens implantation. Quantitative mass spectrometry analysis using spectral counting was performed to determine the relative amounts of hAH proteins in the samples from KC patients and control individuals. RESULTS: All KC patients included in the study presented severe keratoconus (K2 >52 D), and slit-lamp examination revealed microfolds in Descemet’s membrane, without clinical signs of hydrops. We found significant differences between the expression levels of 16 proteins in the two groups. In KC samples, seven proteins were overexpressed and nine were underexpressed in comparison with the control group. Gene ontology analysis revealed that these deregulated proteins are implicated in several biologic processes, such as the regulation of proteolysis, responses to hypoxia, and responses to hydrogen peroxide, among others. CONCLUSIONS: The protein expression profiles in hAH from KC patients and myopic control subjects differ significantly. This result suggests that some components of the hAH proteome are involved in this disease. Further in-depth analysis of the hAH proteome should provide a better understanding of the mechanisms governing the pathophysiology of KC. Molecular Vision 2015-04-25 /pmc/articles/PMC4415580/ /pubmed/25999673 Text en Copyright © 2015 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Soria, Javier Villarrubia, Alberto Merayo-Lloves, Jesús Elortza, Félix Azkargorta, Mikel Alvarez de Toledo, Juan Rodriguez-Agirretxe, Iñaki Suarez, Tatiana Acera, Arantxa Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title | Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title_full | Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title_fullStr | Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title_full_unstemmed | Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title_short | Label-free LC–MS/MS quantitative analysis of aqueous humor from keratoconic and normal eyes |
title_sort | label-free lc–ms/ms quantitative analysis of aqueous humor from keratoconic and normal eyes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415580/ https://www.ncbi.nlm.nih.gov/pubmed/25999673 |
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