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Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide

Direct cell-cell communication through connexin (Cx) complexes is a way to achieve functional accordance of cells within a tissue or an organ. The initial segment (IS), a part of the epididymis, plays important roles in sperm maturation. Steroid hormones influence on expression of a number of genes...

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Autor principal: Lee, Ki-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415647/
https://www.ncbi.nlm.nih.gov/pubmed/25949200
http://dx.doi.org/10.12717/DR.2014.18.4.293
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author Lee, Ki-Ho
author_facet Lee, Ki-Ho
author_sort Lee, Ki-Ho
collection PubMed
description Direct cell-cell communication through connexin (Cx) complexes is a way to achieve functional accordance of cells within a tissue or an organ. The initial segment (IS), a part of the epididymis, plays important roles in sperm maturation. Steroid hormones influence on expression of a number of genes in the IS of adult animals. However, developmental effect of sex hormones on the gene expression in the IS has not been examined. In this study, estradiol benzoate (EB, an estrogen agonist) or flutamide (Flu, an androgen antagonist) was exogenously administrated at 1 week of postnatal age, and expressional changes of Cx genes in the IS were determined at 4 months of age by a quantitative real-time PCR analysis. Treatment of EB at 0.015 μg/kg body weight (BW) increased expression of Cx30.3, 31.1, and 43 genes. However, treatment of 1.5 μg EB/kg BW resulted in expressional decreases of Cx31, 32, and 45 genes and caused increases of Cx30.3 and 43 gene expression. Significant decreases of Cx31, 31.1, 32, 37, and 45 gene expression were detected with a treatment of 500 μg Flu/kg BW, while expression of Cx43 gene was significantly increased with a treatment of 500 μg Flu/kg BW. A treatment of 50 mg Flu/kg BW led to significant increases of Cx30.3, 32, 37, 40, and 43 gene expression. These findings imply that exogenous exposure of steroidal hormones during the early developmental period would result in aberrant expression of Cx genes in the adult IS.
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spelling pubmed-44156472015-05-06 Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide Lee, Ki-Ho Dev Reprod Article Direct cell-cell communication through connexin (Cx) complexes is a way to achieve functional accordance of cells within a tissue or an organ. The initial segment (IS), a part of the epididymis, plays important roles in sperm maturation. Steroid hormones influence on expression of a number of genes in the IS of adult animals. However, developmental effect of sex hormones on the gene expression in the IS has not been examined. In this study, estradiol benzoate (EB, an estrogen agonist) or flutamide (Flu, an androgen antagonist) was exogenously administrated at 1 week of postnatal age, and expressional changes of Cx genes in the IS were determined at 4 months of age by a quantitative real-time PCR analysis. Treatment of EB at 0.015 μg/kg body weight (BW) increased expression of Cx30.3, 31.1, and 43 genes. However, treatment of 1.5 μg EB/kg BW resulted in expressional decreases of Cx31, 32, and 45 genes and caused increases of Cx30.3 and 43 gene expression. Significant decreases of Cx31, 31.1, 32, 37, and 45 gene expression were detected with a treatment of 500 μg Flu/kg BW, while expression of Cx43 gene was significantly increased with a treatment of 500 μg Flu/kg BW. A treatment of 50 mg Flu/kg BW led to significant increases of Cx30.3, 32, 37, 40, and 43 gene expression. These findings imply that exogenous exposure of steroidal hormones during the early developmental period would result in aberrant expression of Cx genes in the adult IS. Korean Society of Developmental Biology 2014-12 /pmc/articles/PMC4415647/ /pubmed/25949200 http://dx.doi.org/10.12717/DR.2014.18.4.293 Text en © Copyright an Official Journal of the Korean Society of Developmental Biology. All Rights Reserve http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Lee, Ki-Ho
Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title_full Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title_fullStr Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title_full_unstemmed Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title_short Expressional Modulation of Connexin Isoforms in the Initial Segment of Male Rat treated with Estradiol Benzoate or Flutamide
title_sort expressional modulation of connexin isoforms in the initial segment of male rat treated with estradiol benzoate or flutamide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415647/
https://www.ncbi.nlm.nih.gov/pubmed/25949200
http://dx.doi.org/10.12717/DR.2014.18.4.293
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