Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection

Porcine circovirus type 2 (PCV2) is a ssDNA virus causing PCV2-systemic disease (PCV2-SD), one of the most important diseases in swine. MicroRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. Viral miRNAs have recently been described and the...

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Autores principales: Núñez-Hernández, Fernando, Pérez, Lester J, Vera, Gonzalo, Córdoba, Sarai, Segalés, Joaquim, Sánchez, Armand, Núñez, José I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416260/
https://www.ncbi.nlm.nih.gov/pubmed/25934266
http://dx.doi.org/10.1186/s13567-015-0181-4
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author Núñez-Hernández, Fernando
Pérez, Lester J
Vera, Gonzalo
Córdoba, Sarai
Segalés, Joaquim
Sánchez, Armand
Núñez, José I
author_facet Núñez-Hernández, Fernando
Pérez, Lester J
Vera, Gonzalo
Córdoba, Sarai
Segalés, Joaquim
Sánchez, Armand
Núñez, José I
author_sort Núñez-Hernández, Fernando
collection PubMed
description Porcine circovirus type 2 (PCV2) is a ssDNA virus causing PCV2-systemic disease (PCV2-SD), one of the most important diseases in swine. MicroRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. Viral miRNAs have recently been described and the number of viral miRNAs has been increasing in the past few years. In this study, small RNA libraries were constructed from two tissues of subclinically PCV2 infected pigs to explore if PCV2 can encode viral miRNAs. The deep sequencing data revealed that PCV2 does not express miRNAs in an in vivo subclinical infection.
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spelling pubmed-44162602015-05-02 Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection Núñez-Hernández, Fernando Pérez, Lester J Vera, Gonzalo Córdoba, Sarai Segalés, Joaquim Sánchez, Armand Núñez, José I Vet Res Short Report Porcine circovirus type 2 (PCV2) is a ssDNA virus causing PCV2-systemic disease (PCV2-SD), one of the most important diseases in swine. MicroRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. Viral miRNAs have recently been described and the number of viral miRNAs has been increasing in the past few years. In this study, small RNA libraries were constructed from two tissues of subclinically PCV2 infected pigs to explore if PCV2 can encode viral miRNAs. The deep sequencing data revealed that PCV2 does not express miRNAs in an in vivo subclinical infection. BioMed Central 2015-05-01 2015 /pmc/articles/PMC4416260/ /pubmed/25934266 http://dx.doi.org/10.1186/s13567-015-0181-4 Text en © Núñez-Hernández et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Núñez-Hernández, Fernando
Pérez, Lester J
Vera, Gonzalo
Córdoba, Sarai
Segalés, Joaquim
Sánchez, Armand
Núñez, José I
Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title_full Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title_fullStr Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title_full_unstemmed Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title_short Evaluation of the capability of the PCV2 genome to encode miRNAs: lack of viral miRNA expression in an experimental infection
title_sort evaluation of the capability of the pcv2 genome to encode mirnas: lack of viral mirna expression in an experimental infection
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416260/
https://www.ncbi.nlm.nih.gov/pubmed/25934266
http://dx.doi.org/10.1186/s13567-015-0181-4
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