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Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater
BACKGROUND: It has been known for years that ethanol is a bio-fuel to replace fossil fuels. The ethanol industry requires the utilization of micro-organisms capable production with stresses. The purpose of present study was to isolate and characterize ethanologenic yeast with high potential applicat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416319/ https://www.ncbi.nlm.nih.gov/pubmed/25937930 http://dx.doi.org/10.1186/2052-336X-12-107 |
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author | Tofighi, Azadeh Mazaheri Assadi, Mahnaz Asadirad, Mohammad Hosein Arash Zare Karizi, Shohreh |
author_facet | Tofighi, Azadeh Mazaheri Assadi, Mahnaz Asadirad, Mohammad Hosein Arash Zare Karizi, Shohreh |
author_sort | Tofighi, Azadeh |
collection | PubMed |
description | BACKGROUND: It has been known for years that ethanol is a bio-fuel to replace fossil fuels. The ethanol industry requires the utilization of micro-organisms capable production with stresses. The purpose of present study was to isolate and characterize ethanologenic yeast with high potential application at high temperature to produce bio-ethanol. METHODS: To isolate ethanologenic yeasts, wastewater samples from a starch producer plant in Varamin, Iran were used. The isolates were identified by molecular characterization. Characteristics of the isolated strains were determined at 30, 35, 40 and 45°C for 48 hours. RESULTS: 50 yeast strains capable of growing well in agar plates in a temperature range of 30–45°C were isolated. Out of the isolated strains, only three strains were screened for their ability to grow at 45°C. Selected yeast, designated as AT-3 strain which showed efficient flocculation capabilities with higher ethanol production and grew faster as compared to the rest of strains in media with 180 g/L glucose at 35°C. The selected yeast was identified as a new strain of Saccharomyces cerevisiae and submitted to the Gene-Bank database. Its’ optimum growth temperature was between 35 and 40°C. The results showed that during the bio-ethanol production 2.5 × 10(10) and 8.5 × 10(9) (CFU/mL) were a good indication of strain capability in heat tolerance. Also, ethanol produced at a raise of 6.9% and 6.85% (w/v) at 35 and 40°C, respectively, whereas glucose-to-ethanol conversion yield was about 75% of the theoretical value. CONCLUSIONS: Results emphasized that the isolated strain identified as Saccharomyces cerevisiae. This specific strain has thermo-tolerant, osmo-tolerant, flocculating capabilities with potential for application in developing a low cost ethanol industry. |
format | Online Article Text |
id | pubmed-4416319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44163192015-05-02 Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater Tofighi, Azadeh Mazaheri Assadi, Mahnaz Asadirad, Mohammad Hosein Arash Zare Karizi, Shohreh J Environ Health Sci Eng Research Article BACKGROUND: It has been known for years that ethanol is a bio-fuel to replace fossil fuels. The ethanol industry requires the utilization of micro-organisms capable production with stresses. The purpose of present study was to isolate and characterize ethanologenic yeast with high potential application at high temperature to produce bio-ethanol. METHODS: To isolate ethanologenic yeasts, wastewater samples from a starch producer plant in Varamin, Iran were used. The isolates were identified by molecular characterization. Characteristics of the isolated strains were determined at 30, 35, 40 and 45°C for 48 hours. RESULTS: 50 yeast strains capable of growing well in agar plates in a temperature range of 30–45°C were isolated. Out of the isolated strains, only three strains were screened for their ability to grow at 45°C. Selected yeast, designated as AT-3 strain which showed efficient flocculation capabilities with higher ethanol production and grew faster as compared to the rest of strains in media with 180 g/L glucose at 35°C. The selected yeast was identified as a new strain of Saccharomyces cerevisiae and submitted to the Gene-Bank database. Its’ optimum growth temperature was between 35 and 40°C. The results showed that during the bio-ethanol production 2.5 × 10(10) and 8.5 × 10(9) (CFU/mL) were a good indication of strain capability in heat tolerance. Also, ethanol produced at a raise of 6.9% and 6.85% (w/v) at 35 and 40°C, respectively, whereas glucose-to-ethanol conversion yield was about 75% of the theoretical value. CONCLUSIONS: Results emphasized that the isolated strain identified as Saccharomyces cerevisiae. This specific strain has thermo-tolerant, osmo-tolerant, flocculating capabilities with potential for application in developing a low cost ethanol industry. BioMed Central 2014-09-25 /pmc/articles/PMC4416319/ /pubmed/25937930 http://dx.doi.org/10.1186/2052-336X-12-107 Text en © Tofighi et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Tofighi, Azadeh Mazaheri Assadi, Mahnaz Asadirad, Mohammad Hosein Arash Zare Karizi, Shohreh Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title | Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title_full | Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title_fullStr | Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title_full_unstemmed | Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title_short | Bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
title_sort | bio-ethanol production by a novel autochthonous thermo-tolerant yeast isolated from wastewater |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416319/ https://www.ncbi.nlm.nih.gov/pubmed/25937930 http://dx.doi.org/10.1186/2052-336X-12-107 |
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