Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S s...

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Detalles Bibliográficos
Autores principales: Oh, Chang Seok, Seo, Min, Hong, Jong Ha, Chai, Jong-Yil, Oh, Seung Whan, Park, Jun Bum, Shin, Dong Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416368/
https://www.ncbi.nlm.nih.gov/pubmed/25925186
http://dx.doi.org/10.3347/kjp.2015.53.2.237
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author Oh, Chang Seok
Seo, Min
Hong, Jong Ha
Chai, Jong-Yil
Oh, Seung Whan
Park, Jun Bum
Shin, Dong Hoon
author_facet Oh, Chang Seok
Seo, Min
Hong, Jong Ha
Chai, Jong-Yil
Oh, Seung Whan
Park, Jun Bum
Shin, Dong Hoon
author_sort Oh, Chang Seok
collection PubMed
description Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
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spelling pubmed-44163682015-05-04 Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb Oh, Chang Seok Seo, Min Hong, Jong Ha Chai, Jong-Yil Oh, Seung Whan Park, Jun Bum Shin, Dong Hoon Korean J Parasitol Brief Communication Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples. The Korean Society for Parasitology and Tropical Medicine 2015-04 2015-04-22 /pmc/articles/PMC4416368/ /pubmed/25925186 http://dx.doi.org/10.3347/kjp.2015.53.2.237 Text en © 2015, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Oh, Chang Seok
Seo, Min
Hong, Jong Ha
Chai, Jong-Yil
Oh, Seung Whan
Park, Jun Bum
Shin, Dong Hoon
Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title_full Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title_fullStr Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title_full_unstemmed Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title_short Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
title_sort ancient mitochondrial dna analyses of ascaris eggs discovered in coprolites from joseon tomb
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416368/
https://www.ncbi.nlm.nih.gov/pubmed/25925186
http://dx.doi.org/10.3347/kjp.2015.53.2.237
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