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Delineation of a frequency-organized region isolated from the mouse primary auditory cortex

The primary auditory cortex (AI) is the representative recipient of information from the ears in the mammalian cortex. However, the delineation of the AI is still controversial in a mouse. Recently, it was reported, using optical imaging, that two distinct areas of the AI, located ventrally and dors...

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Autores principales: Tsukano, Hiroaki, Horie, Masao, Bo, Takeshi, Uchimura, Arikuni, Hishida, Ryuichi, Kudoh, Masaharu, Takahashi, Kuniyuki, Takebayashi, Hirohide, Shibuki, Katsuei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416634/
https://www.ncbi.nlm.nih.gov/pubmed/25695649
http://dx.doi.org/10.1152/jn.00932.2014
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author Tsukano, Hiroaki
Horie, Masao
Bo, Takeshi
Uchimura, Arikuni
Hishida, Ryuichi
Kudoh, Masaharu
Takahashi, Kuniyuki
Takebayashi, Hirohide
Shibuki, Katsuei
author_facet Tsukano, Hiroaki
Horie, Masao
Bo, Takeshi
Uchimura, Arikuni
Hishida, Ryuichi
Kudoh, Masaharu
Takahashi, Kuniyuki
Takebayashi, Hirohide
Shibuki, Katsuei
author_sort Tsukano, Hiroaki
collection PubMed
description The primary auditory cortex (AI) is the representative recipient of information from the ears in the mammalian cortex. However, the delineation of the AI is still controversial in a mouse. Recently, it was reported, using optical imaging, that two distinct areas of the AI, located ventrally and dorsally, are activated by high-frequency tones, whereas only one area is activated by low-frequency tones. Here, we show that the dorsal high-frequency area is an independent region that is separated from the rest of the AI. We could visualize the two distinct high-frequency areas using flavoprotein fluorescence imaging, as reported previously. SMI-32 immunolabeling revealed that the dorsal region had a different cytoarchitectural pattern from the rest of the AI. Specifically, the ratio of SMI-32-positive pyramidal neurons to nonpyramidal neurons was larger in the dorsal high-frequency area than the rest of the AI. We named this new region the dorsomedial field (DM). Retrograde tracing showed that neurons projecting to the DM were localized in the rostral part of the ventral division of the medial geniculate body with a distinct frequency organization, where few neurons projected to the AI. Furthermore, the responses of the DM to ultrasonic courtship songs presented by males were significantly greater in females than in males; in contrast, there was no sex difference in response to artificial pure tones. Our findings offer a basic outline on the processing of ultrasonic vocal information on the basis of the precisely subdivided, multiple frequency-organized auditory cortex map in mice.
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spelling pubmed-44166342016-04-01 Delineation of a frequency-organized region isolated from the mouse primary auditory cortex Tsukano, Hiroaki Horie, Masao Bo, Takeshi Uchimura, Arikuni Hishida, Ryuichi Kudoh, Masaharu Takahashi, Kuniyuki Takebayashi, Hirohide Shibuki, Katsuei J Neurophysiol Sensory Processing The primary auditory cortex (AI) is the representative recipient of information from the ears in the mammalian cortex. However, the delineation of the AI is still controversial in a mouse. Recently, it was reported, using optical imaging, that two distinct areas of the AI, located ventrally and dorsally, are activated by high-frequency tones, whereas only one area is activated by low-frequency tones. Here, we show that the dorsal high-frequency area is an independent region that is separated from the rest of the AI. We could visualize the two distinct high-frequency areas using flavoprotein fluorescence imaging, as reported previously. SMI-32 immunolabeling revealed that the dorsal region had a different cytoarchitectural pattern from the rest of the AI. Specifically, the ratio of SMI-32-positive pyramidal neurons to nonpyramidal neurons was larger in the dorsal high-frequency area than the rest of the AI. We named this new region the dorsomedial field (DM). Retrograde tracing showed that neurons projecting to the DM were localized in the rostral part of the ventral division of the medial geniculate body with a distinct frequency organization, where few neurons projected to the AI. Furthermore, the responses of the DM to ultrasonic courtship songs presented by males were significantly greater in females than in males; in contrast, there was no sex difference in response to artificial pure tones. Our findings offer a basic outline on the processing of ultrasonic vocal information on the basis of the precisely subdivided, multiple frequency-organized auditory cortex map in mice. American Physiological Society 2015-02-18 2015-04 /pmc/articles/PMC4416634/ /pubmed/25695649 http://dx.doi.org/10.1152/jn.00932.2014 Text en Copyright © 2015 the American Physiological Society Licensed under Creative Commons Attribution CC-BY 3.0 (http://creativecommons.org/licenses/by/3.0/deed.en_US) : © the American Physiological Society.
spellingShingle Sensory Processing
Tsukano, Hiroaki
Horie, Masao
Bo, Takeshi
Uchimura, Arikuni
Hishida, Ryuichi
Kudoh, Masaharu
Takahashi, Kuniyuki
Takebayashi, Hirohide
Shibuki, Katsuei
Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title_full Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title_fullStr Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title_full_unstemmed Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title_short Delineation of a frequency-organized region isolated from the mouse primary auditory cortex
title_sort delineation of a frequency-organized region isolated from the mouse primary auditory cortex
topic Sensory Processing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416634/
https://www.ncbi.nlm.nih.gov/pubmed/25695649
http://dx.doi.org/10.1152/jn.00932.2014
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