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UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamli...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416691/ https://www.ncbi.nlm.nih.gov/pubmed/25931105 http://dx.doi.org/10.1107/S1600577515004476 |
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author | Bürck, Jochen Roth, Siegmar Windisch, Dirk Wadhwani, Parvesh Moss, David Ulrich, Anne S. |
author_facet | Bürck, Jochen Roth, Siegmar Windisch, Dirk Wadhwani, Parvesh Moss, David Ulrich, Anne S. |
author_sort | Bürck, Jochen |
collection | PubMed |
description | Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170 nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of α-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality <200 nm compared with an OCD setup adapted to a bench-top instrument, and accelerated data collection by a factor of ∼3. In addition, it permits investigations of low hydrated protein films down to 130 nm using a rotatable sample cell that avoids linear dichroism artifacts. |
format | Online Article Text |
id | pubmed-4416691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-44166912015-05-20 UV-CD12: synchrotron radiation circular dichroism beamline at ANKA Bürck, Jochen Roth, Siegmar Windisch, Dirk Wadhwani, Parvesh Moss, David Ulrich, Anne S. J Synchrotron Radiat Beamlines Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170 nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of α-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality <200 nm compared with an OCD setup adapted to a bench-top instrument, and accelerated data collection by a factor of ∼3. In addition, it permits investigations of low hydrated protein films down to 130 nm using a rotatable sample cell that avoids linear dichroism artifacts. International Union of Crystallography 2015-04-11 /pmc/articles/PMC4416691/ /pubmed/25931105 http://dx.doi.org/10.1107/S1600577515004476 Text en © Jochen Bürck et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Beamlines Bürck, Jochen Roth, Siegmar Windisch, Dirk Wadhwani, Parvesh Moss, David Ulrich, Anne S. UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title | UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title_full | UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title_fullStr | UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title_full_unstemmed | UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title_short | UV-CD12: synchrotron radiation circular dichroism beamline at ANKA |
title_sort | uv-cd12: synchrotron radiation circular dichroism beamline at anka |
topic | Beamlines |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416691/ https://www.ncbi.nlm.nih.gov/pubmed/25931105 http://dx.doi.org/10.1107/S1600577515004476 |
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