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UV-CD12: synchrotron radiation circular dichroism beamline at ANKA

Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamli...

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Autores principales: Bürck, Jochen, Roth, Siegmar, Windisch, Dirk, Wadhwani, Parvesh, Moss, David, Ulrich, Anne S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416691/
https://www.ncbi.nlm.nih.gov/pubmed/25931105
http://dx.doi.org/10.1107/S1600577515004476
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author Bürck, Jochen
Roth, Siegmar
Windisch, Dirk
Wadhwani, Parvesh
Moss, David
Ulrich, Anne S.
author_facet Bürck, Jochen
Roth, Siegmar
Windisch, Dirk
Wadhwani, Parvesh
Moss, David
Ulrich, Anne S.
author_sort Bürck, Jochen
collection PubMed
description Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170 nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of α-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality <200 nm compared with an OCD setup adapted to a bench-top instrument, and accelerated data collection by a factor of ∼3. In addition, it permits investigations of low hydrated protein films down to 130 nm using a rotatable sample cell that avoids linear dichroism artifacts.
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spelling pubmed-44166912015-05-20 UV-CD12: synchrotron radiation circular dichroism beamline at ANKA Bürck, Jochen Roth, Siegmar Windisch, Dirk Wadhwani, Parvesh Moss, David Ulrich, Anne S. J Synchrotron Radiat Beamlines Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170 nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of α-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality <200 nm compared with an OCD setup adapted to a bench-top instrument, and accelerated data collection by a factor of ∼3. In addition, it permits investigations of low hydrated protein films down to 130 nm using a rotatable sample cell that avoids linear dichroism artifacts. International Union of Crystallography 2015-04-11 /pmc/articles/PMC4416691/ /pubmed/25931105 http://dx.doi.org/10.1107/S1600577515004476 Text en © Jochen Bürck et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Beamlines
Bürck, Jochen
Roth, Siegmar
Windisch, Dirk
Wadhwani, Parvesh
Moss, David
Ulrich, Anne S.
UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title_full UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title_fullStr UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title_full_unstemmed UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title_short UV-CD12: synchrotron radiation circular dichroism beamline at ANKA
title_sort uv-cd12: synchrotron radiation circular dichroism beamline at anka
topic Beamlines
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416691/
https://www.ncbi.nlm.nih.gov/pubmed/25931105
http://dx.doi.org/10.1107/S1600577515004476
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